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Determination of urinary metabolite of IBP after oral administration and dermal application to rats  

Min Kyung Jin (계명대학교 공중보건학과)
Cho Young Joo (농업과학기술원 환경생태과)
Cha Chun Geun (계명대학교 공중보건학과)
Publication Information
Journal of Environmental Health Sciences / v.28, no.1, 2002 , pp. 67-77 More about this Journal
Abstract
This study was aimed to determine the urinary metabolite of IBP, one of the organophosphorus pesticides, as the biomarkers of exposure. Urine samples were collected for 24 hours in metabolic cages after oral administration and dermal application of IBP to rats. Identification of the derivatized urinary metabolite was determined by GC/MS and excretion time courses of the urinary metabolite was analyzed by GC/FPD. Urinary metabolite o IBP, diisopropyl phosphorothioate, was detected in rats urine both after oral administration and dermal application of IBP. Parent compound was not detected in the experiment. In GC/MS, the mass spectral confirmation for diisopropyl phosphorothioate ion was identified at m/z 254. Diisopropyl phosphorothioate was excreted within 48 hours and 72 hours after oral administration and dermal application of IBP, respectively. In this study, the same urinary metabolite of IBP was detected both in oral and dermal exposure. Generally, excretion of the urinary metabolite after oral administration was faster than after dermal application. It is suggested that urinary diisopropyl phosphorothioate could be used as the biomarkers of exposure to IBP.
Keywords
IBP; Metabolite; Urine; GC/FPD; GC/MS;
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1 Basack, S. B., Oneto, M. L., Fuchs, J. S., Wood, E. J.and Kesten, E. M : Esterases of Corbicula fluminea as biomarkers of exposure to organophosphorus pesticides. Bull. Environ. Contam. Toxicol., 61, 569-576, 1998   DOI   ScienceOn
2 Shafik, M. T., Bradway, D., Biros, F. J. and Enos, H. F : Characterization of alkylation products of diethyl phosphorothioate. J. of Chromatography A, 778, 151-160, 1997   DOI   ScienceOn
3 Daughton, C. G., Cook, A. M. and Alexander, M : Gas chromatographic determination of phosphoruscontaining pesticide metabolites via benzylation. Analytical Chemistry, 51(12), 1949-1953, 1979   DOI   ScienceOn
4 Bradway, D. E., Moseman, R. and May, R. : Analysis of alkyl phosphates by extractive alkylation. Bull. Environm. Contam. ToxicoI., 26, 520-523, 1981   DOI   ScienceOn
5 Tomlin, C : The pesticide manual. 10th, Crop Protection Publications, U.K., 1994
6 Takade, D. Y., Reynold, J. M. and Nelson, J. H : 1-(4Nitrobenzyl)-3-(4-tolyl)triazene as a derivatizing reagent for the analysis of urinary dialkyl phosphate metabolites of organophosphorus pesticides by gas chromatography. J. Agr. Food Chem., 27(4), 746-753, 1979   DOI
7 Park, S. S., Pyo, H., Lee, K. J., Park, S. J. and Park, T. K : The analysis of common metabolites of organophosphorus pesticides in urine by gas chromatography/mass spectrometry. Bull. Korean Chem. Soc., 19(1), 45-50, 1998
8 Ali, F. A. F. and Fukuto, T. R : Toxicity of O,O,S-trialkyl phosphorothioates to the rat. J. Agr. Food Chem., 33(1), 126-130, 1982   DOI
9 Nauman, C. H : Biomarkers of human exposure to pesticides. ACS, New York, 43-61, 1994
10 Marco, G. J., Simoneaux, B. J., Williams, S. C., Cassidy, J. E., Bissig, R. and Muecke, W : Dermal exposure related to pesticide use. ACS, Washington, D. C., 43-61, 1985
11 Lores, E. M. and Bradway, D. E : Extraction and recovery of organophosphorus metabolites from urine using an anion exchange resin. J. Agr. Food Chem., 25(1), 75-79, 1977   DOI
12 He, F : Biological monitoring of occupational pesticides exposure. Int. Arch. Occup. Environ. Health, 65, S69-S76, 1993   DOI
13 Bradway, D. E. and Shafik. T. M : Malathion exposure studies. Determination of mono- and dicarboxylic acids and alkyl phosphates in urine. J. Agr. Food Chem., 25(6), 1342-1344, 1977   DOI
14 Fest, C. and Schmidt, K. L : The chemistry of organophosphorus. Springer-Verlag, Berlin, 337, 1973
15 Vale, J. A.: Toxicokinetic and toxicodynamic aspects of organophosphorus OP insecticide poisoning. Toxicology Letters, 102, 1998
16 Muan, B. and Sk re, J. U : A method for the determination of the main metabolites of malathion in biological samples. J. Agr. Food Chem., 37(4), 1081-1085, 1989   DOI
17 Churchill II, F. C., Ku, D. N. and Miles, J. W : Gasliquid chromatographic inlet block derivatization of organophosphorus pesticides and related dialkyl phosphorothioates. J. Agr. Food Chem., 26(5), 1108-1112, 1978   DOI
18 Richardson, E. R. and Seiber, J. N : Gas chromatographic determination of organophosphorous insecticides and their dialkyl phosphate metabolites in liver and kidney samples. J. Agr. Food Chem., 41(3), 416-422, 1993   DOI   ScienceOn
19 Serrano, R., L$\pez, F.J., Roig-Navarro and Hem ndez, F : Automated sample clean-up and fractionation of chlorpyrifos, chlorpyrifos-methyl and metabolites in mussels using normal-phase liquid chromatography. J. of Chromatography A, 778, 151-160, 1997   DOI   ScienceOn
20 Aprea, C., Sciarra, G. and Lunghini, L : Analytical method for the determination ofurinary alkylphosphates in subjects occupationally exposed to organophosphorus pesticides and in the general population. J. of Analytical Toxicology, 20, 559-563, 1996   DOI