Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Effect of GM-CSF on the Embryonic Development and the Expression of Implantation Related Genes of Mouse Embryos

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF)의 첨가가 생쥐 수정란의 발생과 착상관련 유전자 발현에 미치는 영향

  • Kim, Dong-Hoon (Medical Science Institute, Eulji General Hospital) ;
  • Ko, Duck-Sung (Medical Science Institute, Eulji General Hospital) ;
  • Lee, Hoi-Chang (Medical Science Institute, Eulji General Hospital) ;
  • Lee, Ho-Joon (Department of Physiology, Eulji University School of Medicine) ;
  • Kang, Hee-Gyoo (Department of Medical Technology, Seoul Health College) ;
  • Kim, Tai-Jeon (Department of Medical Technology, Seoul Health College) ;
  • Park, Won-Il (Department of OB/GYN, Eulji University School of Medicine) ;
  • Kim, Seung-Samuel (Department of OB/GYN, Eulji University School of Medicine)
  • 김동훈 (을지병원 의과학연구소) ;
  • 고덕성 (을지병원 의과학연구소) ;
  • 이회창 (을지병원 의과학연구소) ;
  • 이호준 (을지대학교 의과대학 생리학교실) ;
  • 강희규 (서울보건대학 임상병리과) ;
  • 김태전 (서울보건대학 임상병리과) ;
  • 박원일 (을지대학교 의과대학 산부인과학교실) ;
  • 김세웅 (을지대학교 의과대학 산부인과학교실)
  • Published : 2002.06.30
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Abstract

Objective : The purpose of the current series of experiments were to assess the effect of GM-CSF, as a medium supplement, on the development of mouse embryos and the expression of LIF and IL-1? mRNA. Materials and Methods: Mouse 2-cell embryos were collected from the oviducts of 6 weeks old ICR mice at 48 hours after hCG injection. Embryos were cultured in P-1 medium supplemented with mouse GM-CSF (0, 1, 5, 10 ng/ml). The embryo development to blastocysts and hatching blastocysts was assessed and the cell number in blastocyst was also examined. Using RT-PCR, the expressions of LIF and IL-1? mRNA in blastocyst were evaluated in the GM-CSF supplemented group and control group. Results: In mouse, the addition of GM-CSF increased the percentage of blastocysts (65.5%, 68.6%, 73.0% and 76.1% for control and 1, 5 and 10 ng/ml, respectively), and increased the proportion of hatching blastocysts (35.2%, 36.4%, 43.2% and 53.0% for control and 1, 5 and 10 ng/ml, respectively). The mean cell numbers in blastocyst were significantly increased in GM-CSF supplemented groups compared to control group. LIF and IL-1? expression in blastocyst were significantly higher in GM-CSF supplemented group than in control group. Conclusion: The results of experiment by mouse embryos showed beneficial effects of GM-CSF as a medium supplement. Furthermore, the addition of GM-CSF significantly increased the expression of LIF and IL-1? in mouse embryos. These results suggest that GM-CSF might be a important molecule in embryo implantation.

Keywords

References

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