생물환경조절학회지 (Journal of Bio-Environment Control)

  • 제11권3호
  • /
  • Pages.115-120
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  • 2002
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  • 1229-4675(pISSN)
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  • 2765-3641(eISSN)
한국생물환경조절학회 (The Korean Society for Bio-Environment Control)
권호 표지

스타티스 ‘오션 블루’의 자가영양배양시 광도, 환기횟수 및 $\textrm{CO}_2$ 농도가 소식물체의 기내 생육에 미치는 영향

Autotrophic Growth of Limonium spp. 'Ocean Blue' Plantlets In Vitro as Affected by PPF, NAEH and $\textrm{CO}_2$ Concentration

  • 정기원 (경상대학교 응용생명과학부) ;
  • 정병룡 (경상대학교 농업생명과학연구원)
  • 발행 : 2002.09.01
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초록

자가영양배양시 광도,환기횟수 및 $CO_2$농도가 스타티스 ‘Ocean Blue’ 소식물체의 생장에 미치는 영향을 조사하였다 MS 배지에서 광흔합영양해양을 통하여 증식된 스타티스 식물체에서 2~3장의 잎을 가진 줄기 절편체를 채취하여 배양용기당 4개씩 치상하였다. 절편체는 온도 25$\pm$1$^{\circ}C$. 상대습도 70~ 80%인 배양실에서 cool-white 형광램프로 명기동안 70, 150, 또는 220$\mu$mol. $m^{-2}$ . $s^{-1}$의 광을 공급하면서 41일 동안 배양하였다. MS 기본배지를 사용하였으며, sucrose와 vitamin은 첨가하지 않았다. 광도가 높아질수록 생체 중,건물중 및 엽면적이 증가하였다. $CO_2$농도를 높여준 경우 초장, 생체중, 건물중 및 엽면적이 증가하였다. 뿌리길이와 엽록소 농도는 광도나 $CO_2$의 영향을 받지 않았다. 배양용기 내의 환기횟수를 높이고, 고광도의 $CO_2$를 공급할수록,그리고 PPF를 증가시킬수록 스타티스 소식물체의 자가영양배양에서의 생육이 촉진되었다.

Growth and development of Limonium spp.‘Ocean Blue’plantlets were studied under three levels of photosynthetic photon flux (PPF),70,150 and 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ , two levels of $CO_2$ concentration, 500 and 1000 $\mu$mol. $m^{-1}$ , and two levels of number of air exchanges per hour (NAEH),0.1 $h^{-1}$ and 2.8 $h^{-l}$. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants per vessel were cultured under cool-white fluorescent lamps for 16 h. $d^{-1}$ at 25$\pm$11$^{\circ}C$ and 70~80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter and two $CO_2$ concentrations in the culture rooms were provided from a liquefied $CO_2$ tank. Fresh and dry weights, height, length of the longest root, number of loaves, and leaf area significantly increased with increasing PPF and especially, $CO_2$ concentration. Growth was enhanced by a 2.8 $h^{-1}$ NAEH. Overall, treatment with a 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ PPF and a 1000 $\mu$mol. $m^{-1}$ $CO_2$ resulted in the most vigorous growth of Limonium spp. ‘Ocean Blue’ plantlets.s.

키워드

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