• Title/Summary/Keyword: 미세증식

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Application of Saccharified Acorn-starch for Biomass and Lipid Accumulation of Microalgae (당화된 도토리의 전분이 미세조류 바이오매스 증식과 바이오오일 함량에 미치는 영향)

  • Choi, Hee-Jeong;Lee, Jung-Min
    • Journal of Korean Society on Water Environment
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    • v.32 no.2
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    • pp.197-204
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    • 2016
  • The growth of the algae strain Chlorella vulgaris under mixotrophic conditions in the presence of saccharified acorn-starch (acorn-glucose) was evaluated with the objective of increasing biomass growth and triacylglycerols (TAGs) content. The results indicated that 81.3% of starch was converted to glucose in acorns. C.vulgaris algal strains grown with acorn-glucose produced higher biomass and TAGs content than with autotrophic growth. The highest biomass production and TAGs content with 3 g/L acorn-glucose were 12.44 g/L and 32.9%, respectively. Biomass production with 3 g/L acorn-glucose was 16.4 fold higher than under autotrophic growth condition. These findings suggested that 3 g/L acorn-glucose is economic and efficient for biomass production/productivity and TAGs content of microalgae. This study provides a feasible way to reduce the cost of bioenergy production from microalgae.

핵융합로 증식재용 $\gamma$-$LiAlO_2$분말 합성

  • 박지연;문영태;김원주;오석진;김영석;국일현
    • Proceedings of the Korean Nuclear Society Conference
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    • 1995.05a
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    • pp.747-752
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    • 1995
  • 핵융합로 증식재용 ${\gamma}$-LiAlO$_2$ 분말을 자발착화 연소반응법을 적용하여 합성하였다. LiAlO$_2$분말을 합성하는데 이 방법을 적용하면 다른 분말 합성법과 달리 짧은 시간 내에 미세한 ${\gamma}$상을 쉽게 형성할 수 있었다. 최적의 ${\gamma}$-LiAlO$_2$ 분말을 합성하려면 구연산과 우레아의 혼합연료가 가장 적절하다고 판단되며, 이 연료로 합성된 ${\gamma}$-LiAlO$_2$ 분말을 24시간 볼분쇄하면 비표면적 값이 15.8 m$^2$/g인 미세한 분말을 얻을 수 있었다.

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Effect of Environment Control with Growth Stage on Ex Vitro Plantlets Growth in Photoautotrophic Micropropagation of Potato(Solanum tuberosum L. cv. Dejima) (감자의 광독립영양 미세증식에 있어 생육단계별 환경조절이 기외 생육에 미치는 영향)

  • Son, Jung-Ik;Jang, Il;Lee, Hoon
    • Proceedings of the Korean Society for Bio-Environment Control Conference
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    • 2002.04a
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    • pp.93-96
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    • 2002
  • 감자는 오랜 재배 역사를 가진 세계4대 식량작물로 영양가가 풍부하며 생산량이 어느 작물보다 많기 때문에 전략식량으로 많이 이용되고 있다. 최근 가공산업의 발달로 감자의 수요가 급격히 증가함에 따라 감자의 대량 생산에 관련된 많은 연구들이 수행되어 왔으며, 그 중 미세증식 기법은 단기간에 대량 생산할 수 있다는 점에서 각광을 받고 있다. (중략)

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Effect of Fucoidan on Angiogenesis and Gene Expression in Human Umbilical Vein Endothelial Cells (후코이단이 혈관 내피세포의 신생혈관 생성 효과 및 관련 유전자의 발현에 미치는 영향)

  • Park, Ho;Kim, Beom-Su
    • Korean Journal of Clinical Laboratory Science
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    • v.49 no.4
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    • pp.323-328
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    • 2017
  • Angiogenesis is a process including members of the angiogenic factors. In particular, fibroblast growth factor 2 (FGF2) is considered the most potent angiogenic factor because it promotes cell proliferation and tube formation. A recent study reported that fucoidan derived from marine plant potentiated FGF-2 induced tube formation in human endothelial cells. On the other hand, the molecular mechanisms involved in the angiogenic activity of fucoidan and FGF2 are unknown. In this study, a fucoidan treatment promoted angiogenesis induced by FGF2. The effects of fucoidan on FGF2-induced angiogenesis were confirmed by a proliferation assay using a CellTiter96 Aqueous One solution after a treatment with fucoidan and FGF2. The tube formation and wound healing assay for the angiogenic activity were also confirmed. Reverse transcription PCR showed a change in the mRNA of vascular endothelial growth factor-A (VEGF-A), intercellular adhesion molecule-1 (ICAM-1), matrix metallopeptidase9 (MMP9), and the signal transducer and activator of transcription3 (STAT3). In summary, the Fucoidan/FGF2 treatment induced an increase in cell proliferation, improved the tube formation and wound healing activity, and altered the STAT3, VEGF-A, ICAM-1, and MMP9 mRNA expression levels. Further research will be needed to provide a scientific explanation in terms of cell-signaling and confirm the present findings.

Effect of Vibrio alginolyticus on the Algicidal Activity of Shewanella sp. SR-14 (Vibrio alginolyticus가 Shewanella sp. SR-14의 미세조류 증식저해 활성에 미치는 영향)

  • KIM Ji Hoe;PARK Hee Yeon;LEE Tae Seek;KIM Shin-Hee;PARK Jeong Heum;CHANG Dong Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.5
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    • pp.430-434
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    • 2001
  • The algicidal activity of Shewanella (formerly Alteromonas) sp. SR-14 against diatom, Chaetoceros calcitrans was reported in our previous papers. In this study, the effect of Vibrio alginolyticus on the algicidal activity of Shewanelia sp, SR-14 was examined under the optimum algicidal conditions, i.e., temperature ($21\pm1^{\circ}C$), light intensity (4,000 lux), and light: dark cycle (12 hour: 12 hour). Shewanella sp. SR-14 grew well in the presence or the absence of V. alginolyticus in Conwy medium. Algal growth was only inhibited by Shewanella sp. SR-14. V. alginolyticus did not show the algicidal activity, Growth of C. calcitrans increased synergistically with growth of V. alginolyticus. When the initial inoculum of V. alginolyticus was only 1 log cycle higher than that of Shewanella sp. SR-14, the effect of V. alginolyticus on the algicidal activity of Shewanella sp. SR-14 was insignificant during incubation of mixed culture, i.e., two bacterial species and the alga. However, when V. alginolyticus dominated Shewanella sp. SR-14 by 3 log cycles of bacterial counts, it was found that the strain SR-14 could not inhibited growth of C. calcitrans up to 5 days of incubation.

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Growth of Strawberry Plantlets Cultured in Vitro in the Agar or Commercial Plug Medium as Affected by Ionic Strength (이온강도에 따른 Agar와 공정육묘용 상토에서 기내배양된 딸기 소식물체의 생육)

  • Hwang, Seung-Jae;Jeong, Byoung-Ryong
    • Horticultural Science & Technology
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    • v.30 no.2
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    • pp.201-207
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    • 2012
  • This study was conducted to investigate the effect of supporting material and ionic strength of the MS medium on growth of strawberry plantlets cultured in vitro for the rapid mass production. Explants of $Fragaria$ $ananassa$ 'Houkouwase' in vitro were planted in the agar or Tosilee (commercial plug medium) medium as the supporting material and supplied with 1/4 MS, 1/2 MS or basal (as the control) MS medium in an autotrophic micropropagation. Plant height and root length were significantly greater when they were cultured in 1/2 MS medium as compared to those grown in the agar medium. Also, shoot fresh and dry weights, and leaf area in the 1/2 MS medium were greater than in 1/4 MS or basal MS medium. When plantlets were cultured in Tosilee medium and fed with the basal MS medium, plant height, root length, shoot fresh and dry weights, root fresh and dry weights, and leaf area were promoted and greater than those in plantlets cultured in the agar medium.

Effect of explant's position and culture method on shoot proliferation and micro-cuttings for a rare and endangered species, Abeliophyllum distichum Nakai (희귀 및 멸종위기 식물 미선나무(Abeliophyllum distichum Nakai)의 절편위치 및 치상방법에 따른 기내증식 및 미세삽목)

  • Lee, Na Nyum;Kim, Ji-Ah;Kim, Yong-Wook;Choi, Yong Eui;Moon, Heung Kyu
    • Journal of Plant Biotechnology
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    • v.42 no.3
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    • pp.228-234
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    • 2015
  • Using either the apical or axillary bud of the endangered species Abeliophyllum distichum Nakai, we tested the effect of bud position and culture method on shoot proliferation and rooting. In shoot proliferation, the axillary bud explant was more effective than the apical bud and the effect was fostered by BA treatment, whereas no differences were observed in shoot elongation by the explant position. Spontaneous rooting was observed in the MS basal medium and resulted in conspicuous differences in the explant position : more than 80% in apical bud explant and 28% in axillary bud explant was achieved, respectively. The positional effects were also observed in BA pre-treatments: generally vertical culture method appeared to be better in shoot proliferation, growth, and rooting than that of the horizontal culture method regardless of the BA pre-treatment duration. The highest shoot multiplication was achieved through the vertical culture method with axillary bud explant, whereas the best shoot elongation and rooting was obtained using the vertical culture method with the apical bud explant. Apical bud explant was superior to axillary bud explant in ex vitro micro-cuttings and revealed a significant difference in shoot growth and root development. The above results suggest that explant position and culture method influence the efficiency of micropropagation for a rare and endangered plant Abeliophyllum distichum.