한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제47권1호
  • /
  • Pages.62-67
  • /
  • 2002
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  • 0252-9777(pISSN)
  • /
  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
권호 표지

Microspore Division and Plant Regeneration from Shed Pollen Culture in Rice

  • Kim, Hyun-Soon (National Honam Agricultural Experiment Station, RDA) ;
  • Kang, Hyeon-Jung (Division of Research Coordination, RDA) ;
  • Lee, Young-Tae (National Honam Agricultural Experiment Station, RDA) ;
  • Lee, Seung-Yeob (Division of Plant Resources Science, Wonkwang University) ;
  • Nam, Jeong-Kwon (National Honam Agricultural Experiment Station, RDA) ;
  • Kim, Tae-Soo (National Honam Agricultural Experiment Station, RDA) ;
  • Rha, Eui-Shik (Research Center for Industrial Development of Bio Food Materials. Chonbuk National University) ;
  • Jin, Il-Doo (Coll. Of Agri. And Life Science, Sunchon national University)
  • 발행 : 2002.03.01
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초록

An efficient system of rice microspore culture could contribute to the production of genetically modified rice. The microspores were isolated by mechanical or shed methods. The number of microspores per 100 anthers isolated at uninucleate stage was higher than (or similar to) those at binucleate stage in isolation method with pestle or spatular, but microspore divisions were not easily observed on both stages. On the other hand, pollen division in shed pollen culture was observed more frequently at uninuclear than at binuclear stage. Cold pretreatment at 1$0^{\circ}C$ for 10 days resulted in the best multicellular division to produce microcalli at 12.5% efficiency in shed microspores. Heat shock at 33$^{\circ}C$ for one hour before or after pollen shedding enhanced cell division and callus formation. Out of twelve green regenerants, two were haploids and ten were diploids based on the chromosome analysis of root tips. The size of stoma was 12$^{m}$ m in haploids and 15 ${\mu}{\textrm}{m}$ in diploids determined by scanning electron microscope (SEM).

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