Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
권호 표지

The Evaluation of Estrogenic/Antiandrogenic Activity of Puerariae Radix in Immature Rats Using Uterotrophic Assay and Hershberger Assay

랫드에서 Uterotrophic assay 및 Hershberger assay를 이용한 칡의 에스트로겐/항안드로겐 영향 평가

  • 곽승준 (국립독성연구원 특수독성부) ;
  • 김순선 (국립독성연구원 특수독성부) ;
  • 이규식 (국립독성연구원 특수독성부) ;
  • 손경희 (국립독성연구원 특수독성부) ;
  • 김희연 (식품의약품안전청 식품평가부) ;
  • 강길진 (식품의약품안전청 식품평가부) ;
  • 최요우 (국립독성연구원 특수독성부) ;
  • 박철훈 (국립독성연구원 특수독성부) ;
  • 박귀례 (국립독성연구원 특수독성부)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out to evaluate the ostrogenic/antiandrogenic activity of Puerariae Radix in Sprague-Dawley rats. It has known that diverse phytoestrogen were included in some Puerariae Radix, especially in Pueraria mirifica. The Uterotrophic assay and Hershberger assay were performed to evaluate the ostogenic/antiandrogenic activity of various Puerariae Radix (Pueraria thunbergiana, Pueraria mirifica and Butea superba). In Uterotrophic assay, the extracts of Puerariae Radix were administered subcutaneously to immature female SD rats from 19 to 21 days of age. The wet uterus and vaginal weighs significantly increased in the group only treated with extracts of Pueraria mirifica. But, in Hersh-berger assay, all extracts of Puerariae Radix did not show any effects in the castrated rats. These results suggest that Pueraria mirifica has not undrogenic/antiandrogenic effect but potent estrogenic effect. It is possible that components of Pueraria mirifica may act as endocrine disruptor in human body.

Keywords

References

  1. J. Nat. Prod. v.63 Identification of deoxy miroestrol as the actual rejuvenating principle of Kwao Keur. Pueraria mirifica. The known miroestrol may be and frtifact Chansakaow,S.;Ishikawa,T.;Seki,H.;Sekine,K.;Okada,M.;Chaichantipyuth,C. https://doi.org/10.1021/np990547v
  2. Planta Med. v.66 Isoflavonoides from Pueraria mirifica and their estrogenic activity Chansakaow,S.;Ishikawa,T.;Sekine,K.;Okada,M.;Higuchi,Y.;Kudo,M.;Chaichantipyuth,C. https://doi.org/10.1055/s-2000-8603
  3. J. Toxicol. Pub. Health v.16 Uterotrophic activity of ethinyl estradiol by gavage and subcutaneous administration in immature female rats Chung,M.K.;Lim,K.H.;Kim,J.C.;Kim,Y.H.;Suh,J.E.;Ha,C.S.
  4. J. Toxicol. Pub. Health v.16 The antiandrogenic effects of di(n-butyl) phthalate in immature male rats:Establishment of Hershberger assay for endocrine disruptors Chung,M.K.;Kim,J.C.;Suh,J.E.
  5. US Environmental Protection Agency 'Integrated risk information system' on-line EPA
  6. Pro. Soc. Exp. Biol. Med. v.83 Myotrophic activity of 19-nortestosterone and other steroids determined by modified levator ani muscle method Hershberger,L.;Shipley,E.;Meyer,R. https://doi.org/10.3181/00379727-83-20301
  7. J. Am. Diet. Assoc. v.95 Urinary isoflavonoid phytoestrogen and lighan excretion after consumption of fermented and unfermented soy products Hutchins,A.M.;Slavin,J.L.;Lampe,J.W. https://doi.org/10.1016/S0002-8223(95)00149-2
  8. Environ. Health Perspect v.104 no.SUP.4 Research needs for the risk assessment of health and environmental effects of endocrine disruptors:a report of the U.S. EPA-sponsered workshop Kavlock,R.J.;Daston,G.P.;DeRosa,D.;Fenner Crisp.P.;Gary,L.E.;Kaattari,S.;Lucier,G.;Luster,M. https://doi.org/10.1289/ehp.96104s4715
  9. J. Toxicol. Pub. Health v.16 Evaluation of in vitro and invivo screening methods for estrogenic activity of endocrine disruptors Kim,H.S.;Han,S.Y.;Han,S.K.;Shin,J.H.;Moon,H.J.;Kim,S.H.;Park,K.S.;Kim,K.B.;Lee,R.D.;Jang,S.J.;Park,K.L.
  10. Validation management committe:Endocrine disruptors screening and testing, Agenda Item 5, Valdation protocol for the uterotrophic assay OECD
  11. J. Steroid Biochem. Mol. Biol. v.48 The effect of androgens and antiandrogens on the immunohistochemical localization of the androgen receptor in accessory reproductive organs of mail rats. Paris,F.;Weinbauer,G.F.;Blum,V.;Nieschlag,E. https://doi.org/10.1016/0960-0760(94)90259-3
  12. Fundam. Appl. Toxicol. v.34 Survey and assessment of mammalian estrogen biological assays for hazard characterization Reel,J.R.;Lamp,J.C.;Neal,B.H. https://doi.org/10.1006/faat.1996.0198
  13. Lancet v.350 Exposure of infants to phyto-oestrogens from soy-based infant formula Setchell,K.D.;Zimmer Nechemias,L.;Cai,J.;Heubi,J.E. https://doi.org/10.1016/S0140-6736(96)09480-9
  14. J. Med. Chem. v.18 Synthesis and biological activity of some esters of testosterone Solo,A.J.;Bejba,N.;Hebborn,P.;May,M. https://doi.org/10.1021/jm00236a011