한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제22권4호
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  • Pages.273-278
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  • 2002
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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이탈리안 라이그라스 X 톨페스큐 속간 교잡종의 약배양 기법을 이용한 식물체 생산

Production of New Regenerated Plants by Anther Culture of the Hybrids of Italian ryegrass X Tall fescue

  • Kim, K.Y. (National Livestock Research Institute) ;
  • Kang, K.M. (National Livestock Research Institute) ;
  • Choi, K.J. (National Livestock Research Institute) ;
  • Jang, Y.S. (National Livestock Research Institute) ;
  • Lim, Y.C. (National Livestock Research Institute) ;
  • Kim, M.J. (National Livestock Research Institute) ;
  • Kim, J.G. (National Livestock Research Institute) ;
  • Kim, W.H. (National Livestock Research Institute) ;
  • Park, G.J. (National Livestock Research Institute)
  • 발행 : 2002.12.01
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초록

이탈리안 라이그라스 (Lolium multinorum Lam.)와 톨페스큐 (Festuca arundinacea Schreb.)의 속간 교잡종 3계통에 대한 약 배양을 실시하여 약 유래의 식물체를 생산하였다. MS 기본배지에 sucrose 30 g/$\ell$, NAA 2 mg/$\ell$, kinetin 1 mg/$\ell$을 첨가한 캘러스 유도배지에서 배양 20일만에 약 유래의 캘러스를 유도하였다. 캘러스 유도율은 평균 11.6%였으며, 캘러스의 크기는 평균 9.1 mg/callus/anther 였다. MS 기본배지에 sucrose 30 g/$\ell$ , NAA 1 mg/$\ell$, kinetin 2 mg/$\ell$을 첨가한 재분화 배지에 캘러스를 치상하여 배양 50일만에 완전히 재분 화된 식물체를 얻었으며, 캘러스로부터 식물체로 재분화 되는 비율은 평균 27.1%였고, Hyb-1 계통의 식물체 재분화율이 30.2%로서 가장 높았다.

Several regenerated plants were obtained from anther culture of the hybrid lines of Italian ryegrass (Lolium multiflorum Lam.) X Tall fescue (Festuca arundinacea Schreb.). When the anthers of the hybrid plants were incubated for 20 days on callus induction medium, MS medium containing 30 g/$\ell$ of sucrose, 2 mg/$\ell$ of NAA and 1 mg/$\ell$ of kinetin, their calli were induced. The mean ratio of callus induction was 11.6 percent, and the mean of callus weight was 9.1 mg/callus/anther. When the calli of the hybrid plants were incubated for 50 days on plant regeneration medium, MS medium containing 30 g/$\ell$ of sucrose, 1 mg/$\ell$ of NAA and 2 mg/$\ell$ of kinetin, the hybrid plants were regenerated. The mean ratio of plant regeneration was 27.1 percent and line Hyb-1 showed highest regenerabillity with the frequency of 30.2 percent.

키워드

참고문헌

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피인용 문헌

  1. Determination of Heat Killing Temperature of Birdsfoot trefoil and Italian ryegrass vol.24, pp.4, 2004, https://doi.org/10.5333/KGFS.2004.24.4.341