Degradation Characteristics of Methyl Ethyl Ketone and Methyl Isobuthyl Ketone by Pseudomonas putida KT-3.

Pseudomonas putida KT-3의 Methyl Ethyl Ketone 및 Methyl Isobuthyl Ketone 분해 특성

  • 김민주 (바이오세인트㈜ 환경생명공학연구소) ;
  • 이태호 (바이오세인트㈜ 환경생명공학연구소) ;
  • 이경미 (바이오세인트㈜ 환경생명공학연구소) ;
  • 류희욱 (바이오세인트㈜ 환경생명공학연구소, 숭실대학교 환경 화학공학과) ;
  • 조경숙 (이화여자대학교 환경학과)
  • Published : 2002.12.01

Abstract

Methyl ethyl ketone (MEK) and methyl isobutyl ketone (MIBK) have been widely used as solvents in various industries. Biodegradation of MEK and MIBK by Pseudomonas putida KT-3, which could utilize MEK or MIBK as a sole carbon source, was characterized, and the cosubstrate interaction in MEK/MIBK mixture was also studied. Within the range of initial MEK concentration (from 0.5 to 5.5 mM), an increased substrate concentration increased the specific degradation rate of MEK by P putida KT-3 (from 3.15 to 10.58 mmol/g DCW$\cdot$h), but the rate sightly increased at 11.0 mM of initial MEK concentation (11.28 mmol/g DCW$\cdot$h). The similar degradation rates of MIBK (4.69-4.92 mmol/g DCW$\cdot$h) were obtained at more than 3.0 mM of initial MIBK concentation. Kinetic analysis on the degradation of MEK/MIBK mixture by P. putida KT-3 showed that MEK or MIBK acted as a competitive inhibitor. Maximum degradation rate ($V_{max}$), saturation constant ($K_{m}$) and inhibition constant ($K_{1}$) were as follows: $V_{max,MEK}$=12.94 mmol/g DCW$\cdot$h; $K_{m,MEK}$=1.72 mmol/L; $K_{l,MEK}$=1.30 mmol/L; $V_{max,MIBK}$=5.00 mmol/g-DCW$\cdot$h; $K_{m,MIBK}$=0.42 mmol/L; $K_{l,MEK}$=0.77 mmol/L.

각종 산업시설에서 유기용제로 사용되는 methyl ethylketone(MEK)과 methyl isobutyl ketone(MIBK)을 유일 탄소원으로 이용할 수 있는 Pseudomonas putida KT-3 균주에 의한 이들 물질의 생분해 특성을 조사하였고, MEK/MIBK 혼합물 분해에 미치는 이들 기질 상호간의 작용을 규명하였다. MEK 단독 기질 조건에서 MEK 첨가농도가 0.5에서 5.5mM로 증가함에 따라 KT-3 균주에 의한 MEK 분해속도도 3.15에서 10.58 mmol/g DCW$\cdot$h로, MEK 첨가량이 5.5mM인 경우와 거의 유사한 속도를 얻을 수 있었다. 또한 MIBK 단독 기질 조건에서 KT-3 균주에 의한 MIBK 분해속도는 3.0mM 이상의 MIBK 농도에서는 MIBK 농도에 상관없이 4.69-4.96 mmol/gDCW$\cdot$h로 거의 일정하였다. KT-3 균주의 MEK/MIBK 혼합물에서의 생분해 속도의 감소는 두 기질 상호간의 경쟁적인 저해작용에 의한 것임을 알 수있었고, 속도론적 해석 결과 얻는 MEK와 MIBK의 최대분해속도 ($V_{max}$), 포화상수 ($K_{m}$) 및 저해상수 ($K_{1}$)는 다음과 같다. $V_{max,MEK}$=12.94 mmol/g DCW$\cdot$h; $K_{m,MEK}$=1.72 mmol/L; $K_{l,MEK}$=1.30 mmol/L; $V_{max,MIBK}$=5.00 mmol/g-DCW$\cdot$h; $K_{m,MIBK}$=0.42 mmol/L; $K_{l,MEK}$=0.77 mmol/L.

Keywords

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