Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

A Rapid Detection of Methicillin-Resistant Staphylococci by Polymerase Chain Reaction

Polymerase Chain Reaction을 이용한 Methicillin-resistant Staphylococci의 신속 검출

  • 박진숙 (한남대학교 이과대학 미생물학과) ;
  • 박영진 (한남대학교 이과대학 미생물학과)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

PCR of the mecA gene for the rapid detection of methicillin-resistant staphylococci was perfomed and compared with the antibiotic sensitivity test. A total of 43 strains of staphylococi from clinical specimens were used in this study. An antibiotic sensitivity test by the agar dilution method of NCCLS (The National Commitee for Clinical Laboratory Standard) was performed for the strains. Among them, 39 isolates were methicillin-resistant (MRS), and 4 isolates were methicillin-susceptible (MSS). With the exception for one strain (Staphylococcus cohnii, HRC2-4), all MRS strains amplified the expected 533 bp fragments of the mecA gene by PCR, However, one strain (Staphylococcus aureus, HSA1-10) that was classified as a sensitive strain by the antibiotic sensitivity test was mecA positive by PCR. All 35 methicillin-resistant Staphylococcus aureus (MRSA) strains were mecA positive, but overall, concordance between the results of the mecA PCR and antibiotic sensitivity test was 95.6%.

Methicillin-resistant staphylococci를 신속하게 검출하기 위한 방법으로 mecA polymerase chain reaction을 이용하고 결과를 항생제 감수성 검사와 비교하였다. 국내 병원에 입원중인 환자와 종사자로부터 총 43개의 staphylococci를 분리하여 항생제 감수성 시험을 실시한 결과, 43균주 중 methicillin 내성 균주(MRS)는 39 균주, 감수성 균주(MSS)는 4균주이었다. PCR시험결과, methicillin 내성 균주에서는 MRSC (methicillin-resistant Staphylococcus cohnii), 1 균주(HRC2-4)를 제외하고 mecA 유전자에 해당하는 533bp의 절편이 중폭되었다. 한편, methicillin 감수성으로 분류된 MSSA (methicillin-susceptable Staphylococcus aureus), 1 균주(HSA1-10)에서도 mecA 유전자가 중폭되어 항생제 감수성과 상반된 결과를 보였다. 모든 MSSA는 mecA 양성을나타내었으나, 전체적으로 mecA 유전자의 유무는 항생제 감수성 검사와 95.6%의 일치를 나타냈다.

Keywords

References

  1. 감염 v.27 Methicillin 내성 황색 포도상구균의 각종 항균제에 대한 내성 양상과 plasmid DNA의 특성 유필열;김영일;'이준행;정선식;안태휴;신종희;양동욱;김영휴
  2. Antimicrob. Agents Chemother. v.34 Detection of methicillin resistance in staphylococci by using a DNA probe Archer, G.L.;E. Pennel
  3. J. Clin. Microbiol. v.35 Comparison and application of ribosome spacer DNA-amplicon polymorphism and pulsed-field gel elecrophoresis for differentitaion of methicillin-resistant Staphyococcus aureus strains Deplano, A.;M. Vaneechoutte;G. Verschraegen;M.J. Struelens
  4. J. Clin. Microbiol. v.40 Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): A disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test Felten A.;B. Grandry;P.H. Lagrange;I. Casin
  5. J. Clin. Microbiol. v.36 Comparison of ATB Staph, rapid ATB Staph, Vitek, and E-test methods for detection of oxacillin heteroresistance in staphylococci possesing mecA Frebourg, N.B.;D. Nouet;L. Lemee;E. Martin;J.F. Lemeland
  6. J. Clin. Microbiol. v.35 Typing of Staphylococcus aureus and Staphylococcus epidermidis strains by PCR analysis of inter-IS256 spacer length polymorphisms Hookey, J.V.;J.F. Richardson;B.D. Cookson
  7. J. Clin. Microbiol. v.40 Rapid detection of methicillin in coagulase-negative staphylococci with the Vitek 2 system Horstkotte, M.A.;J.K.-M. Knobloch;H.Rohde;S. Dobinsky;D. Mack
  8. J. Clin. Microbiol. v.37 Comparison of susceptibility testing method with mecA gene analysis for determining oxacillin resistance in clinical isolates of Staphylococcus aureus and coagulase-negative Staphylococcus spp. Kumari, D.N.;V. Keer;P.M. Hawkey;P.parnell;N. Joseph;J.F. Richardson;B. Cookson
  9. J. Mol. Biol. v.3 A procedure for the isolation of deoxyribonucleic acid from microorganisms Marmur, J.
  10. J. Clin. Microbiol. v.37 Evalution of MRSA-screen, a simple Anti-PBP 2a slide latex agglutination kit for rapid detection of methicillin resistance in Staphylococcus aureus Matthias, C.;A. Wenger;K. Jaaton;D.S. Blanc;J. Bille
  11. Antimicrob. Agents Chemother v.31 Effects of temperature, NaCl and methicillin on penicillin-binding proteins, growth, peptidoglycan synthesis and autolysis in methicillin-resistant Staphylococcus aureus Murty, V.;D.P. Brunner;B.J. Wilkinson
  12. Antimicrob. Agents Chemother v.32 Development of enzyme-labeled oligonucleotide probe for detection of mecA gene in methicillin-resistant Staphlylococcus aureus Shimaoka, M.;M. Yoh;A segawa;Y. Takarada;K. Yamoto;T. Honda
  13. FEBS Lett. v.221 Evolution of an inducible penicillin target protein in methicillin-resistant S. aureus by gene fusion Song, M.D.;M. Wachi;M. Doi;F. Ishino;M. Matsuhashi
  14. J. Clin. Microbiol. v.38 Rapid detection of mecA-positive and mecA-negative staphylococci by an anti-penicillin binding protein 2a slide latex agglutination test Stoakes, H.Z.L.;S. Garrow;S. Longo;V. Fitzgerald;R. Lannigan
  15. J. Clin. Microbiol. v.39 Comparison of the Vitek Gram-positive susceptobility 106 card, the MRSA-screen latex agglutination test, and mecA analysis for detecting oxacillin resistance in a geographically diverse collection of clinical isolates of coagulase-negative staphylococci Yamazumi, T.;I. Fruta;D.J. Diekema;M.A. Pfaller;R.N. Jones