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A Rapid Detection of Methicillin-Resistant Staphylococci by Polymerase Chain Reaction  

박진숙 (한남대학교 이과대학 미생물학과)
박영진 (한남대학교 이과대학 미생물학과)
Publication Information
Korean Journal of Microbiology / v.38, no.4, 2002 , pp. 306-311 More about this Journal
Abstract
PCR of the mecA gene for the rapid detection of methicillin-resistant staphylococci was perfomed and compared with the antibiotic sensitivity test. A total of 43 strains of staphylococi from clinical specimens were used in this study. An antibiotic sensitivity test by the agar dilution method of NCCLS (The National Commitee for Clinical Laboratory Standard) was performed for the strains. Among them, 39 isolates were methicillin-resistant (MRS), and 4 isolates were methicillin-susceptible (MSS). With the exception for one strain (Staphylococcus cohnii, HRC2-4), all MRS strains amplified the expected 533 bp fragments of the mecA gene by PCR, However, one strain (Staphylococcus aureus, HSA1-10) that was classified as a sensitive strain by the antibiotic sensitivity test was mecA positive by PCR. All 35 methicillin-resistant Staphylococcus aureus (MRSA) strains were mecA positive, but overall, concordance between the results of the mecA PCR and antibiotic sensitivity test was 95.6%.
Keywords
mecA gene; MRS; PCR; Staphylococci;
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