Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Organization of Antibiotic Resistance Gene Cluster of Multi-Drug Plasmid in Clinically Isolated Salmonella Enteritidis Strain

식중독환자에서 분리한 Salmonella Enteritidis 다제내성 플라스미드의 내성유전자 집락의 구조해석

  • Jung, Seo-Yeon (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Son, Chang-Kyu (Kyungsangbuk-do Institute of Health and Environment) ;
  • Kwak Kyung-Tak (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Byung-Chun (Department of Microbiology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Wan (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
  • 정서연 (경북대학교 자연과학대학 미생물학과) ;
  • 손창규 (경상북도 보건환경연구원) ;
  • 곽경탁 (경북대학교 자연과학대학 미생물학과) ;
  • 김병천 (경북대학교 자연과학대학 미생물학과) ;
  • 박완 (경북대학교 자연과학대학 미생물학과)
  • Published : 2002.12.01
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Abstract

Clinically isolated Salmonella Enteritidis strain has a multi drug resistance plasmid, which confers ampicillin, chloramphe-nicol, sulfonamide, streptomycin and tetracycline, named pCAST2. We cloned a 7 kb Sacl fragment of pCAST2 which has sulfonamide, streptomycin and tetracycline resistance genes. The 7 kb SacI fragment showed the organization of sulII-strA-strB-tetR-tetA gene cluster which is different from the other clusters reported previously. In this study, we presented the method to detect this cluster by PCR analysis and showed that this cluster was found in Salmonella strains occurred sporadically at Kyungpook province in 2002.

2001년 경북지역의 설사환자로부터 분리된, Salmonella Enteritidis SY9 균주로부터 ampicillin, chloramphenicol, sulfonamide, streptomycin, tetracycline에 내성을 가지는 40 kb plasmid를 분리하였다. pCAST2 라고 명명한 이 다제내성 plasmid로부터 sulfonamide, streptomycin, tetracycline 내성유전자를 가지는 약 7 kb의 Sacl 단편을 클로닝 하였다. 이 7 kb 단편 염기서열의 내성유전자의 구조는 sulII-strA-strB-tetR-tetA의 집락으로 기존에 보고되지 않은 새로운 유전자 배열을 나타내었다. 본 연구에서는 내성유전자 집락을 검출할수 있는 primer를 제작하여 PCR 분석을 통해서 이들 구조를 탐지할 수 있는 방법을 제시하였다. 또한 PCR 분석을 통한 구조 비교로, 이 집락이 2002년 8월에 경북지역에서 산발적으로 발생한 Salmonella 균주에서도 발견됨을 확인하였다.

Keywords

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