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Organization of Antibiotic Resistance Gene Cluster of Multi-Drug Plasmid in Clinically Isolated Salmonella Enteritidis Strain  

Jung, Seo-Yeon (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
Son, Chang-Kyu (Kyungsangbuk-do Institute of Health and Environment)
Kwak Kyung-Tak (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
Kim, Byung-Chun (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
Park, Wan (Department of Microbiology, College of Natural Sciences, Kyungpook National University)
Publication Information
Korean Journal of Microbiology / v.38, no.4, 2002 , pp. 299-305 More about this Journal
Abstract
Clinically isolated Salmonella Enteritidis strain has a multi drug resistance plasmid, which confers ampicillin, chloramphe-nicol, sulfonamide, streptomycin and tetracycline, named pCAST2. We cloned a 7 kb Sacl fragment of pCAST2 which has sulfonamide, streptomycin and tetracycline resistance genes. The 7 kb SacI fragment showed the organization of sulII-strA-strB-tetR-tetA gene cluster which is different from the other clusters reported previously. In this study, we presented the method to detect this cluster by PCR analysis and showed that this cluster was found in Salmonella strains occurred sporadically at Kyungpook province in 2002.
Keywords
pCAST2; PCR; Salmonella Enteritidis; sulII-strA-strB-tetR-tetA cluster;
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