KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Development of a Flow Injection Analysis Technique for On-line Monitoring of Xylitol Concentrations

자일리톨 농도의 온라인 모니터링을 위한 흐름주입분석기술 개발

  • 이종일 (전남대학교 응용화학공학부, 생물공정기술연구실)
  • Published : 2002.08.01
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Abstract

Flow injection analysis technique for monitoring of xylitol concentrations in biological processes has been developed using xylitol oxidase (XYO) immobilized on VA-Epoxy Biosynth carrier. The immobilized XYO cartridge has been integrated into a FIA system with an oxygen electrode and systematically investigated with regards to the factors which can affect the activity of the immobilized XYO, such as pH, temperature, salt concentration etc. The activity of the immobilized XYO increased with the temperature ($19.0 - 29.0^{circ}C$) and sample injection volume ($75-250\muL$) and molarity of potassium phosphate buffer (0.1-1 M), but it reached the highest value at pH 8.5. The XYO-FIA system has been also applied for on-line monitoring of xylitol concentrations in a reactor and showed good operational stability and agreement with off-line data measured with HPLC.

Keywords

References

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