Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Apicularen A, a Macrolide from Chondromyces sp., Inhibits Growth Factor Induced In Vitro Angiogenesis

  • Kwon, Ho-Jeong (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University) ;
  • Kim, Dong-Hoon (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University) ;
  • Shim, Joong-Sub (Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University) ;
  • Ahn, Jong-Woong (Korea Research Institute of Chemical Technology)
  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Apicularen A (Api A) was recently isolated from Chondromyces sp. as a potent antitumor agent. Because of its unique chemical structure, a macrolide with a highly unsaturated amide side chain, and potent growth inhibitory effect in various cancer cell lines, Api A is currently in clinical trial for cancer therapy. In the present study, the effect of Api A on in vitro angiogenesis of bovine aortic endothelial cells (BAECS) was investigated. Api A potently inhibited the proliferation of BAECS in a dose-dependent manner. Treatment of the endothelial cells with up to 10 ng/ml of the compound did not show any cytotoxicity. In addition, it inhibited basic fibroblast growth factor (bFGF)-induced invasion and capillary tube formation of BAECS at concentrations of 2-5 ng/ml. These results, therefore, demonstrate that Apl A is a novel antiangiogenic agent and may suppress the growth of tumors, at least in part, by the inhibition of neovascularization.

Keywords

References

  1. J. Nat. Prod. v.62 KR-025, a new cytotoxic compound from Myxococcus fulvus Ahn, J. W.;S. H. Woo;C. O. Lee;K. Y. Cho;B. S. Kim https://doi.org/10.1021/np9804233
  2. J. Pharmacol. Exp. Ther. v.297 Discovery of a novel antitumor benzolactone enamide class that selectively inhibits mammalian vacuolar type (H+) ATPases Boyd, M. R.;C. Farina;P. Belfiore;S. Gagliardi;J. W. Kim;Y. Hayakawa;J. A. Beutler;T. C. McKee;B. J. Bowman;E. J. Bowman
  3. Int. J. Biol. Markers v.24 Endostatin: a promising drug for antiangiogenic therapy Cirri, L.;S. Donnini;L. Morbidelli;P. Chiarugi;M. Ziche;F. Ledda
  4. J. Org. Chem. v.62 Salicylihalamides A and B, novel cytotoxic macrolides from the marine sponge Haliclona sp. Erickson, K. L.;J. A. Beutler;J. H. Cardellian Ⅱ;M. R. Boyd https://doi.org/10.1021/jo971556g
  5. Cell v.86 Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis Hanahan, D.;J. Folkman https://doi.org/10.1016/S0092-8674(00)80108-7
  6. Int. J. Biol. Markers v.14 Generation of expression plasmids for angiostatin, endostatin, and TIMP-2 for cancer gene therapy Indraccolo, S.;S. Minuzzo;E. Gola;W. Habeler;F. Carrozzino;D. Noonan;A. Albini;L. Santi;A. Amadori;L. Chieco-Bianchi
  7. Nature v.348 Synthetic analogues of fumagillin that inhibit angiogenesis and suppress tumor growth Ingber, D.;T. Fujita;S. Kishimoto;K. Sudo;T. Kanamaru;H. Brem;J. Folkman https://doi.org/10.1038/348555a0
  8. J. Biol. Chem. v.275 Canstatin, a novel matrix-derived inhibitor of angiogenesis and tumor growth Kamphaus, G. D.;P. C. Colorado;D. J. Panka;H. Hopfer;R. Ramchandran;A. Torre;Y. Maeshima;J. W. Mier;V. P. Sukhatme;R. Kalluri https://doi.org/10.1074/jbc.275.2.1209
  9. J. Microbiol. Biotechnol. v.11 Anti-angiogenic activity of acalycixenolide E, a novel marine natural product from Acalycigorgia inermis Kwon, H. J.;J. H. Kim;H. J. Jung;Y. G. Kwon;M. Y. Kim;J.-R. Rho;J. Shin
  10. Int. J. Cancer v.97 Histone deacetylase inhibitor FK228 inhibits tumor angiogenesis Kwon, H. J.;M. S. Kim;M. J. Kim;H. Nakajima;K. W. Kim https://doi.org/10.1002/ijc.1602
  11. J. Antibiot. v.51 Apicularen A and B, new cytostatic macrolides from Chondromyces species (Myxobacteria): Production, physico-chemical and biological properties Kunze, B.;R. Jansen;F. Sasse;G. Hofle;H. Reichenbach https://doi.org/10.7164/antibiotics.51.1075
  12. Proc. Natl. Acad. Sci. USA v.98 Endothelial cell surface F1-F0 ATP synthase is active in ATP synthesis and is inhibited by angiostatin Moser, T. L.;D. J. Kenan;T. A. Ashley;J. A. Roy;M. D. Goodman;U. K. Misra;D. J. Cheek;S. V. Pizzo https://doi.org/10.1073/pnas.131067798
  13. J. Microbiol. Biotechnol. v.10 Sclerotiorin and Isochromophilone Ⅳ: Inhibitors of Grb2-Shc interaction, isolated from Penicillium multicolor F1753 Nam, J. Y.;K. H. Son;H. K. Kim;M. Y. Han;S. U. Kim;J. O. Choi;B. M. Kwon
  14. Eur. J. Pharmacol. v.241 Radicicol, a microbial cell differentiation modulator, inhibits in vivo angiogenesis Oikawa, T.;H. Ito;H. Ashino;M. Toi;T. Tominaga;I. Morita;S. Murota https://doi.org/10.1016/0014-2999(93)90206-W
  15. Biochem. Biophys. Res. Commun. v.181 Eponemycin, a novel antibiotic, is a highly powerful angiogenesis inhibitor Oikawa, T.;M. Hasegawa;M. Shimamura;H. Ashino;S. Murota;I. Morita https://doi.org/10.1016/0006-291X(91)92046-M
  16. Chem. Biol. v.27 Myxobacteria-bacteria forming fruiting body Yamanaka, S.