Hepatoprotective Effects of Amorphous and Nnno-Particle Pyeparations of Ursodeoxycholic Acid in CC4-Induced Mice : Effects of Three Types of Fine Grinding Mills

Ursodeoxycholic acid의 무정형 초미립자제제들의 CC4 유도 간손상 생쥐에 대한 보호 효과

  • 정한영 (부산대학교 분체공학협동과정) ;
  • 곽신성 (부산대학교 분체공학협동과정) ;
  • 김현일 (부산대학교 분체공학협동과정) ;
  • 최우식 (부산대학교 분체공학협동과정) ;
  • 이지현 (부산대학교 약학대학) ;
  • 김애라 (부산대학교 약학대학) ;
  • 박태현 (부산대학교 약학대학) ;
  • 정해영 (부산대학교 약학대학) ;
  • 김유정 (부산여자대학 피부미용과)
  • Published : 2002.03.01


The particle size of medicinal materials is an Important physical property that affects the phar-maceutical behaviors such as dissolution, chemical stability, and bioavailability of solid dosage forms. The size reduction of raw medicinal powder is needed to formulate insoluble drugs or slightly soluble medicines and to improve the pharmaceutical properties such as the solubility, the pharmaceutical mixing, and the dispersion. The objective of the present study is to evaluate physiological activity of amorphous and nano-particle prep-arations of insoluble drug, ursodeoxycholic acid (UDCA), which were made by three types of fine grinding mills. The change of physical properties of ground UDCA was conformed by Mastersiger microplus and X-ray diffraction. We have investigated hepatoprotective effects of the nano-particle preparations of UDCA by plan-etary mill, vibration rod mill and jet mill in $CCI_4$-induced oxidatively injured mouse liver. The results showed that nano-particle preparations of UDCA all decreased reactive oxygen sepecies generation and lipid peroxi-dation in $CCI_4$-induced oxidative stress mice. Among them, nano-particle preparations by vibration rod mill and jet mill showed more significantly hepatoprotective effects compared to intact UDCA and planetary mill-ground UDCA. These results suggest that ground UDCA with vibration rod mill and jet mill shows a high amorphous state and the improved dissolution.



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