Browse > Article

Hepatoprotective Effects of Amorphous and Nnno-Particle Pyeparations of Ursodeoxycholic Acid in CC4-Induced Mice : Effects of Three Types of Fine Grinding Mills  

정한영 (부산대학교 분체공학협동과정)
곽신성 (부산대학교 분체공학협동과정)
김현일 (부산대학교 분체공학협동과정)
최우식 (부산대학교 분체공학협동과정)
이지현 (부산대학교 약학대학)
김애라 (부산대학교 약학대학)
박태현 (부산대학교 약학대학)
정해영 (부산대학교 약학대학)
김유정 (부산여자대학 피부미용과)
Publication Information
Biomolecules & Therapeutics / v.10, no.1, 2002 , pp. 1-6 More about this Journal
The particle size of medicinal materials is an Important physical property that affects the phar-maceutical behaviors such as dissolution, chemical stability, and bioavailability of solid dosage forms. The size reduction of raw medicinal powder is needed to formulate insoluble drugs or slightly soluble medicines and to improve the pharmaceutical properties such as the solubility, the pharmaceutical mixing, and the dispersion. The objective of the present study is to evaluate physiological activity of amorphous and nano-particle prep-arations of insoluble drug, ursodeoxycholic acid (UDCA), which were made by three types of fine grinding mills. The change of physical properties of ground UDCA was conformed by Mastersiger microplus and X-ray diffraction. We have investigated hepatoprotective effects of the nano-particle preparations of UDCA by plan-etary mill, vibration rod mill and jet mill in $CCI_4$-induced oxidatively injured mouse liver. The results showed that nano-particle preparations of UDCA all decreased reactive oxygen sepecies generation and lipid peroxi-dation in $CCI_4$-induced oxidative stress mice. Among them, nano-particle preparations by vibration rod mill and jet mill showed more significantly hepatoprotective effects compared to intact UDCA and planetary mill-ground UDCA. These results suggest that ground UDCA with vibration rod mill and jet mill shows a high amorphous state and the improved dissolution.
nano-particle; ursodeoxycholic acid; vibration rod mill; jet mill; hepatoprotective effect;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Esterbauer, H., Schaur, R. J. and H. Zoliner, 1991. Chemistry and Biochemistry of 4-Hyroxynonena1, Malonaldehyde and Related Aldehydes; Free Rad. Biot. Med., 11, 81-128   DOI   ScienceOn
2 Halliwell, B. and J. M. Gutteridge, 1989. Free radicals in biology and medicine. Oxford. Conference
3 Higashi, T, 1988. Critical review on the determination of glutathione in biological preparations, Proteins, Nucleic Acid and Enzyme, 33, 1370-1382
4 Kim, K. R., Kang, C. M., Beak, J. H., Lee, Y. M. and K. W. Kim, 1998. Bile-acid induced apoptosis in HL-60 leukemia cells. J. Kor. Associ. Cancer Preven., 3, 107-114
5 Ursodiol for the long-term treatment of primary biliary cirrhosis. N. Engl. J. Med., 330, 1342-1347
6 Sedlak, J. and R.H. Lindsay, 1968. Estimation of total Protein-bound and nonpropein-bound sulfhydryl groups in tissue with Ellman's reagent. Anal. Biochem., 25, 192-196
7 Yonemochi, E., Inoue, Y., Buckton, G., Moffat, A., Oguchi, T. and K. Yamamoto, 1999. Differences in crystallization behavior between quenched and ground amorphous ursodeoxycholic acid. Pharm. Res., 16, 835-840   DOI   ScienceOn
8 Corfran, R. S. and S. L. Robbins, 1989. Robbins pathologic basis of disease. W. B. saunders, Philadelphia
9 Kulesar-Gergely, J. and A. Kluesar, 1997. Studies in the effects of ursodeoxycholic acid in rats with acute Carbon-tetrachloride injury. Arzneim.-Forsch./Drus Res., 47, 659-661
10 Kwak, S. S., Kim, H. I., Chung, H. Y., Chung, H. Y., Yamamoto, K., Oguchi, T., Tozuka, Y., Yonemoch, E., Terada, K. and W. S. Choi, Amorphous and nano-particle preparation for improvement of insoluble drugs bioavail-ability. 2nd Asian Aerosol
11 Leuschner, U. and W. Kurtz, 1990. Pharmacological aspects and therapeutic effects of ursodeoxycholic acid. Dig. Dis., 8, 12-22   DOI
12 Miller, P., Weiss, S., Cornell, M., J. Dockery, 1981. Specific $^{125}I$-radioinmiunoassay for cholyglycine, a bile acid, in serum, Clin Chem., 27, 1698-1703
13 Mitsuyoshi, H., Nakashima, T., Sumida, Y, Yoh, T., Nakajima, Y, Ishikawa, H., Inaba, K., Sakamoto, Y., Okanoue, T. and K. Kashima, 1999. Ursodeoxycholic acid protects hepato-cytes against oxidative injury via induction of antioxidants. Biochem. Biophys. Res. Commun., 263, 537-542   DOI   ScienceOn
14 Nakagawa, S., Makino, I., Ishizaki, T. and I. Dohi, 1977. Dissolution of cholesterol gallstones by ursodeoxycholic acid. Lancet, 20, 367-369
15 Poupon, R. E., Poupon, R., Balkau, B. N. and The UDCA-PBC Study Group. 1994