Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Effects of Pinus densiflora Sieb. et Zucc. Ethanol Extract on Listeria monocytogenes

소나무(Pinus densiflora Sieb. et Zucc.) 에탄올 추출물의 Listeria monocytogenes에 대한 항균효과

  • 임용숙 (대구가톨릭대학교 식품공학과) ;
  • 배만종 (경산대학교 식품과학과) ;
  • 이신호 (대구가톨릭대학교 식품공학과)
  • Published : 2002.04.01
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Abstract

To develope food preservative, antimicrobial activities of Pinus densiflora (PD) ethanol extract against Listeria monocytogenes Scott A. Listeria monocytogenes Brie I and Listeria monocytogenes ATCC 19111 were investigated. The ethanol extracts of PD showed strong antimicrobial activities on Listeria monocytogenes. The crude ethanol extracts of PD were further fractionated by ether, ethyl acetate and butanol. The ether fraction from ethanol extract showed the strongest antimicrobial effects on Listeria monocytogenes in tryptic soy broth containing 40 mg/mL ether fractions compared with other fractions. The effect of ethanol extract of pinus densiflora against Listeria monocytogenes culture for growth stage in tryptic soy broth at 35$^{\circ}C$ showed the strongest antimicrobial activites for lag phase. The morphological changes of the cells were observed with transmission electron microscope (TEM) and scanning electron microscope (SEM) and the cells were injured by treatment of 40 mg/mL ethanol extract of Pinus densiflora.

소나무를 95% 에탄올로 추출하여 얻은 추출물을 40 mg/mL 첨가하였을때 Listeria monocytogenes Scott A, Listeria monocytogenes Brie I. isteria monocytogenes ATCC 19111 3균주의 성장이 억제되었으며 이를 다시 용매별로 순차 분획한 후 3균주에 대한 증식저해효과는 특히 ether 분획 물이 높게 나타났다. 소나무 추출물 40 mg/mL 첨가에 의해 Listeria monocytogenes 3균주 모두 균체의 성장 단계 중 유도기인 4시간째 회수한 균체에서 높은 생균수 감소 현상이 확인되었다. 전자현미경으로 확인한 균체의 형태는 대조구의 균체표면은 매끈하고 손상의 흔적이 없으나 처리구의 균체의 표면은 손상되었거나 형태가 이상을 일으킨 모습을 볼 수 있었다.

Keywords

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