Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Green Tea (-)EGCG Induces the Apoptotic Death of Lung Cancer Cells via Activation of c-Jun N-terminal Kinase 1 and Activating Protein-1

녹차의 (-)EGCG에 의한 사람 폐암 세포주 A549의 c-Jun N-terminal Kinase 1과 Activating Protein-1활성화를 통한 세포고사

  • 박지선 (전북대학교 식품영양학과) ;
  • 신미경 (원광대학교 식품영양학과) ;
  • 손희숙 (전북대학교 식품영양학과) ;
  • 박래길 (원광대학교 의과대학 미생물학교실) ;
  • 김명선 (원광대학교 의과대학 미생물학교실) ;
  • 정원훈 (원광보건대학)
  • Published : 2002.01.01
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Abstract

Green tea has been recognized as a favorite beverage for centuries in Easter and Westers cultures. Recently, anti-tumor effects of green tea constituents have received increasing attention. However, the mechanism of catechin-mediated cytotoxicity against tumor cells remains to be elusive. To elucidate the mechanical insights of anti-tumor effects, (-)epigallocatechin-gallate(EGCG) of catechin was applied to human lung cancer A549 cells. (-)EGCG induced the death of A549 cells, which was revealed as apoptosis in DNA fragmentation assay. (-)EGCG induced the activation of caspase family cysteine proteases including capase-3, -8 and -9 proteases in A549 cells. Furthermore, (-)EGCG increased the phosphotransferase activity of c-Jun N-terminal kinase 1JNK 1), which further induced tole transcriptional activation of activating protein-1(AP-1) in A549 cells. We suggest that (-)EGCG-induced apotosis of A549 cells is mediated by signaling pathway involving caspase family cysteine protease, JNK1 and transcription factor, AP-1.

Keywords

References

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