Determination of Phthalates in Biota Samples Using Gas Chromatography/Mass Spectrometry

기체크로마토그래피/질량분석법을 이용한 생체시료 중 프탈산 에스텔류의 분석법

  • Seo, Jung-Ju (Hazardous Substance Research Team, Korea Basic Science Institute) ;
  • Na, Yuncheol (Hazardous Substance Research Team, Korea Basic Science Institute) ;
  • Hong, Jongki (Hazardous Substance Research Team, Korea Basic Science Institute)
  • 서정주 (한국기초과학지원연구원, 유해물질분석팀) ;
  • 나윤철 (한국기초과학지원연구원, 유해물질분석팀) ;
  • 홍종기 (한국기초과학지원연구원, 유해물질분석팀)
  • Received : 2001.09.12
  • Published : 2001.10.25

Abstract

A method for the analysis of most common phthalate acid esters (9 secies) in biota samples by gas chromatography-mass spectrometry-selected ion monitoring mode is described. Phthalates in biota samples are extracted by organic solvent and purified by Florisil column. Phthalates are easily contaminated during extraction prodedure. Since the extraction and cleanup steps for biota samples generally are more complicate than those for water or sediment samples, we compared with contamination state of each sample work-up step. By applying this developed method, the overall recoveries ranged between 79 - 117% in biota sample which was spiked with standards. For phthalates used in this study, the quantitaive accuracy, elution pattern on Florisil column, and detection limits were also investigated.

본 연구는 프탈산 에스텔류에 대하여 생물시료 중에 존재하는 가장 일반적인 9종의 분석을 기체크로마토그래피-질량분석기-선택이온검색법에 의해 수행하였다. 생물시료는 유기용매로 추출하고 Florisil 컬럼을 사용하여 정제하였다. 프탈산 에스텔류는 전처리 과정 중 가장 쉽게 오염되는 물질로 생물시료의 경우 전처리 단계가 수질이나 저질시료보다 복잡해지므로 각 전처리 단계의 오염정도를 비교 분석하였다. 표준물질이 첨가된 생물시료에 본 연구에서 개발된 분석법을 적용하여 구한 회수율은 79 - 117%정도로 나타났다. 또한 프탈산 에스텔류의 정량의 정확도, Florisil 컬럼 용리패턴, 검출한계 등을 조사하였다.

Keywords

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