Animal cells and systems

  • 제5권4호
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  • Pages.327-331
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  • 2001
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Effects of Polychlorinated Biphenyls on the Expression of KAP3 Gene Involved in the 'Critical Period' of Rat Brain Sexual Differentiation

  • Lee, Chae-Kwan (Institute of Industrial Medicine, Inje University) ;
  • Kang, Han-Seung (Department of Biology, College of Bioengineering, Inje University) ;
  • June, Bu-ll (Department of Biology, College of Bioengineering, Inje University) ;
  • Lee, Byung-Ju (Department of Biology, College of Natural Sciences, Ulsan University) ;
  • Moon, Deog-Hwan (Institute of Industrial Medicine, Inje University) ;
  • Kang, Sung-Goo (Department of Biology, College of Bioengineering, Inje University)
  • 발행 : 2001.12.01
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초록

There is a critical developmental period during which brain sexual differentiation proceeds irreversibly under the influence of gonadal hormone. Recently, kinesin superfamily-associated protein 3 (KAP3) gene expressed during the 'critical period' of rat brain differentiation was identified by us (Choi and Lee, 1999). KAP3 functions as a microtubule-based motor that transports membranous organelles anterogradely in cells, including neurons (Yamazaki et al., 1996). mRNA level of KAP3 gene markedly increased before the initiation of puberty. Neonatal treatment of estrogen clearly inhibited the prepubertal increase in KAP3 mRNA level (Choi and Lee, 1999). In the present study, we aimed to investigate the effects of polychlorinated biphenyls (PCBs), as endocrine disruptors (EDs) on the expression of KAP3 gene during the 'critical period' of rat brain development. In our data, PCBs significantly decreased the expression of KAP3 gene in the fetal (day 17) and the neonatal (day 6 after birth in) male and female rat brains. The body weight and the breeding ability were significantly decreased in the PCBs-exposed rats compared with the control. These results showed that PCBs affect the transcriptional level of brain sexual differentiation related gene, KAP3, in the fetal and the neonatal rat brains. The maternal exposure to the PCBs may lead to toxic response in embryonic brain sexual differentiation and breeding ability after sexual maturation. This study indicates that KAP3 gene may be useful as a gene marker to analyze the molecular mechanism of toxic response in the animal brain development and sexual maturation exposed to PCBs.

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참고문헌

  1. Barraclough CA (1961) Production of anovulatory, sterile rats by single injections of testosterone propionate. Endocrinology 68: 62-67
  2. Barraclough CA (1979) Sexual differentiation of cyclic gona dotropin secretion. Adv Biosci 25: 433-450
  3. Brady ST (1985) A novel brain ATPase with properties expected for the fast axonal transport motor. Nature 317: 73-75 https://doi.org/10.1038/317073a0
  4. Choi EJ and Lee BJ (1999) Identification of genes involved in the onset of female puberty of rat. Korean J Biol Sci 3: 319-329
  5. Dohler KD (1998) Influence of hormones and hormone antagonists on sexual differentiation of the brain. Arch Toxicol Suppl 20: 131-141
  6. Faber KA, Ayyash L, and Dixon S (1993) Effect of neonatal diethylstilbestrol exposure on volume of the sexually dimorphic nucleus of the preoptic area of the hypothalamus and pituitary responsiveness to gonadotroin-releasing hormone in female rats of known anogenital distance at birth. Biol Reprod 48: 947-951 https://doi.org/10.1095/biolreprod48.5.947
  7. Faber KA and Hughes CL (1991) The effect on neonatal exposure to DES, genistein, and zearalenone on pituitary responsiveness and SDN-POA volume in the castrated adult rat. Biol Reprod 45: 649-653 https://doi.org/10.1095/biolreprod45.4.649
  8. Fielden MR, Halgren RG, Tashiro CH, Yeo BR, Chittim B, Chou K, and Zacharewski TR (2001) Effects of gestational and lactational exposure to Aroclor 1242 on sperm quality and in vitro fertility in early adult and middle-aged mice. Reprod Toxicol 15: 281-292 https://doi.org/10.1016/S0890-6238(01)00129-0
  9. Gallant TL, Singh A, and Chu I (2000) PCB 118 induces ultra structural alterations in the rat liver. Toxicology 145: 127-134 https://doi.org/10.1016/S0300-483X(00)00141-4
  10. Gerall AA, Hendricks SE, and Johnson LL (1967) Effects of early castration in male rats adult sexual behavior. J Comp Physiol 64: 206-212
  11. Gorski RA (1963) Modification of ovulatory mechanisms by postnatal administration of estrogen to the rat. Am J Physiol 205: 842-844
  12. Gorski RA (1985) Sexual dimorphisms of the brain. J Anim Sci 61: 38-61
  13. Greenough WT, Carter CS, and Steerman C (1977) Sex differences in dentritic patterns in hamster preoptic area. Brain Res 126: 63-72 https://doi.org/10.1016/0006-8993(77)90215-3
  14. Hayashi S and Aihara M (1989) Neonatal estrogenization of the female rat: a useful model for analysis of hypothalamic involvement of gonadotropin release. Zool Sci 6: 1059-1068
  15. hayashi S, Aihara M, and Wakabayashi K (1991) Content and disribution pattern of luteinizing hormone-releasing hormone (LHRH) in the hypothalamus of neonatally estrogenized female rats. Neurosci Res 12: 366-378 https://doi.org/10.1016/0168-0102(91)90004-I
  16. Hirokawa N, Sato-Yoshitake R, and Kobayashi N (1991) Kinesin associates with anterogradely transported membranous organelles. in vivo. J cell Biol 114: 295-302 https://doi.org/10.1083/jcb.114.2.295
  17. Hirokawa N (1993) Axonal transports and the cytoskeleton. Curr Opin Neurobiol 3: 724-731 https://doi.org/10.1016/0959-4388(93)90144-N
  18. Hirokawa N (1997) The mechanisms of fast and slow transport in neurons: identification and characterization of the new kinesin superfamily motors. Curr Opin Neurobiol 7: 605-614 https://doi.org/10.1016/S0959-4388(97)80079-7
  19. Kester MH, Bulduj S, and Tibboel D (2000) Potent inhibition of estrogen sulfotransferase by hydroxylated PCB metabolites: a novel pathway explaining the estrogenic activity of PCBs. Endocrinology 141: 1897-1900 https://doi.org/10.1210/en.141.5.1897
  20. Matsumoto A and Arai Y (1981) Neuronal plasticity in the differentiated hypothalamic arcuate nucleus of adult female rats and its enhancement by treatment with estrogen. J Comp Neurol 197: 197-205 https://doi.org/10.1002/cne.901970203
  21. McEwen BS, Jones KJ, and Pfaff DW (1987) Hormonal control of sexual behavior in the female rat: molecular, cellular and neurochemical studies. Biol Reprod 36: 37-45 https://doi.org/10.1095/biolreprod36.1.37
  22. Mousa MA, Quensen JF, and Chou K (1966) Microbial dechlorination alleviates inhibitory effects of PCBs on mouse gamete fertilization in vitro. Environ Sci Technol 30: 2087-2092 https://doi.org/10.1021/es950929x
  23. Ness DK, Schantz SL, and Moshtaghian J (1993) Effects of prenatal exposure to specific PCB congeners on thyroid hormone concentrations and thyroid histology in the rat. Toxicol Lett 68: 311-323 https://doi.org/10.1016/0378-4274(93)90023-Q
  24. Palanza P, Parmigiani S, and Liu H (1999) Prenatal exposure to low doses of the estrogenic chemicals diethylstillbestrol and o,p-DDT alters aggressive behavior of male and female house mice. Pharmacol Biochem Behav 64: 665-672 https://doi.org/10.1016/S0091-3057(99)00151-3
  25. Parsons B, MacLusky NJ, and Krey L (1980) The temporal relationship between estrogen-inducible progestin receptors in the female rat brain and the time course of estrogen activation of mating behavior. Endocrinology 107: 774-779
  26. Pinilla L, Trimino E, and Garnelo P (1993) Changes in pituitary secretion during the early postnatal period and anovulatory syndrome induced by neonatal oestrogen or androgen in rats. J Reprod Fertil 97: 13-20 https://doi.org/10.1530/jrf.0.0970013
  27. Raisman G and Field PM (1973) Sexual dimorphism in the neuropil of the preoptic area of the rat and its dependence on neonatal androgen. Brain Res 54: 1-29 https://doi.org/10.1016/0006-8993(73)90030-9
  28. Rice DC and Hayward S (1999) Effects of exposure to 3,3',4,4'5-pentachlorobiphenyl (PCB 126) throughout gestation and lactation on behavior (concurrent random interval-random interval and progressive ratio performance) in rats. Neurotoxicol Teratol,21: 679-687 https://doi.org/10.1016/S0892-0362(99)00021-5
  29. Simerly RB, Zee MC, and Phedleton JW (1997) Estrogen receptor-dependent sexual differentiation of dopaminergic neurons in the preoptic region of the mouse. Proc Natl Acad Sci USA 94: 14077-14082 https://doi.org/10.1073/pnas.94.25.14077
  30. Toran-Aller CD (1984) Gonadal hormones and brain development: implications for the genesis of sexual differentiation. Ann NY Acad Sci 435: 101-111 https://doi.org/10.1111/j.1749-6632.1984.tb13743.x
  31. Vale RD, Reese TS, and Sheetz MP (1985) Identification of a novel force-generating protein, kinesin, involved in micro-tubule-based motility. Cell 42: 39-50 https://doi.org/10.1016/S0092-8674(85)80099-4
  32. vom Saal FS and Bronson FH (1980) Sexual characteristics of adult female mice are correlated with aystheir blood testosterone levels during prenatal development. Science 208: 597-599 https://doi.org/10.1126/science.7367881
  33. Weisz J and Ward IL (1980) Plasma testosterone and progesterone titers of pregnant rats, their male and female fetuses, and neonatal offspring. Endocrinology 106: 306-316
  34. Yamazaki H, Nakada T, and Okada Y (1995) KIF3A/B: A heterodimeric kinesin superfamily protein that works as a microtuble plus end-directed motor for membrane organelle transport. J Cell Biol 130: 1387-1399 https://doi.org/10.1083/jcb.130.6.1387
  35. Yamazaki H, Nakata T, and Okada Y (1996) Cloning and characterization of KAP3: a novel kinesin superfamily associated protein of KIF3A/3B. Proc Natl Acad Sci USA 93: 8443-8448 https://doi.org/10.1073/pnas.93.16.8443