초록
육계를 13주간 사육하면서 5주령부터 시험사료를 급여한 후 세포표면항원의 발현과 탐식세포에서 생성되는 $O_{2}$ -와 $H_{2}$ $O_{2}$ 에 미치는 영향 그리고 항원 특이적인 항체 생성에 미치는 영향을 측정하기 위하여 아마종실과 항산화제 무첨가구 ($T_{1}$), 아마종실만을 혼합한 사료 ($T_{2}$), 아마종실에 ;$\alpha$ -tocopherol 첨가구 ($T_{3}$ )와 아마종실에 $\alpha$ -tocopherol과 셀레늄 첨가구 ($T_{4}$ )를 육계에 급여한 바, 시험사료를 2주간 급여시 호중구 및 macrophages와 같은 탐식 세포의 $O_{2}$ -와 $H_{2}$ $O_{2}$ 생성 능력은 시험구 ($T_{2}$ , $T_{3}$ ,$T_{4}$ )들이 $T_{1}$ 구보다 증가하였으나 유의차는 없었다. 시험사료를 4주간 급여시 $O_{2}$ -와 $H_{2}$ $O_{2}$ 생성 능력은 $T_{2}$ 구와 $T_{1}$ 구간 큰 차이는 없었으나 $T_{3}$ 구와 $T_{4}$ 구의 $O_{2}$ -생성능력은 $T_{1}$ 구보다 각각 6%와 18%가 증가하였고, $H_{2}$ $O_{2}$ 생성 능력은 각각 9.5%와 10.9%가 증가하였다. 시험사료를 8주간 급여한 후 $T_{3}$ 와 $T_{4}$ 구의 $O_{2}$ -생성능력은 $T_{1}$ 구에 비하여 50%가 넘은 유의성 (P<0.01) 있는 강력한 증가가 있었고, $H_{2}$ $O_{2}$ 생성능력은 각각 6.6%와 9.3%가 증가하였다.
To examine the effects of feed additives on the expression of Perpheral blood cell surface molecules, phagocytosis and antigen specific antibody formation, broilers were randomly assigned to T$_1$, T$_2$, T$_3$, and T$_4$ groups. T$_1$ group was fed diet without any additives for 13 weeks, T$_2$ was fed diet with full fat flax, T$_3$ was fed diet with full fat flax containing $\alpha$-tocopherol, and T$_4$ was fed diet with full-fat flax containing $\alpha$-tocopherol and selenium. Since 5 weeks feeding the data were examined by luminometer. After 2 weeks adminstration of different feeding, although all treated groups (T$_2$, T$_3$, and T$_4$,) showed slightly increased chemiluminescence (CL) responses than T$_1$, this result was not significant. After 4 weeks feeding there was no significant increase of CL in the Phagocytes like neutrophils and macrophages of T$_2$ group compared to T$_1$. But phagocytes from T$_3$ and T$_4$ group showed in creased $O_2$- (6%, 18% respectively) as well as $H_2O$$_2$ (9.5% and 10.9%, respectively) induced CL responses. After 8 weeks feeding there was more than 50% increase $O_2$- induced CL in T$_3$ and T$_4$ group, but $H_2O$$_2$ induced CL responses in T$_3$ and T$_4$ group was slightly increased (6.6% and 9.3%, respectively).
