Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Immunohistochemical Study of the Expression of Bone Morphogenetic Protein(BMP-7) Following Regenerative Periodontal Surgery

생쥐 치아의 초기발생과정에서 Osteopontin mRNA의 발현

  • Han, Kyung-Yoon (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Cho, Se-Yeol (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Lim, ki-Jungl (Department of Periodontology, College of Dentistry, Chosun University) ;
  • Kim, Heung-Jung (Department of Oral Anatomy, College of Dentistry, Chosun University) ;
  • Park, Ju-Cheol (Department of Oral Histology, College of Dentistry, Chosun University) ;
  • Kim, Byung-Ock (Department of Periodontology, College of Dentistry, Chosun University)
  • 한경윤 (조선대학교 치과대학 치주과학교실) ;
  • 조세열 (조선대학교 치과대학 치주과학교실) ;
  • 임기정 (조선대학교 치과대학 치주과학교실) ;
  • 김흥중 (조선대학교 치과대학 구강해부학교실) ;
  • 박주철 (조선대학교 치과대학 구강조직학교실) ;
  • 김병옥 (조선대학교 치과대학 치주과학교실)
  • Published : 2000.03.30
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Abstract

Extracellular matrix component is degraded by enzymes of thematrix metalloproteinases(MMPs). MMPs are produced by both hemopoietic and structural cells. Increased activity of MMP-3 in periodontium is strongly associated with inflammatory periodontal disease. The purpose of the present study was to estimate the effect of BMP-7 on regeneration of periodontium. The optical density was measured by microwell plate reader at 450 nm.The difference of the optical density and the relative activity ofMMP-3 according to the concentration were statistically analyzed by one way ANOVA. The results were as follows: 1. Tetracycline-HCl showed the tendency to inhibit the activity of MMP-3 at the concentration lower than $25{\mu}g/ml$. 2. Doxycycline-HCl inhibited significantly the activity of MMP-3 at the concentration lower than $100{\mu}g/ml$. 3 . Minocycline-HCl inhibited the activity of MMP-3 at the concentration in the range of 10 to 200${\mu}g/ml$. Within the limit of the present study, the above results suggested that bone morphogenetic protein-7 may play a important role in development of periodontium.

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