Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
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Antibacterial Effects and Cytotoxicity of Crassirhizomae Rhizoma

관중의 항균작용 및 세포독성에 관한 연구

  • Kim, Seung-Nam (Department of Periodontology, College of Dentintry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, College of Dentintry, Seoul National University) ;
  • Rhyu, In-Cheol (Department of Periodontology, College of Dentintry, Seoul National University) ;
  • Hahm, Byung-Do (Department of Periodontology, College of Dentintry, Seoul National University) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Han, Soo-Boo (Department of Periodontology, College of Dentintry, Seoul National University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, College of Dentintry, Seoul National University) ;
  • Choi, Sang-Mook (Department of Periodontology, College of Dentintry, Seoul National University)
  • 김승남 (서울대학교 치과대학 치주과학교실) ;
  • 구영 (서울대학교 치과대학 치주과학교실) ;
  • 류인철 (서울대학교 치과대학 치주과학교실) ;
  • 함병도 (서울대학교 치과대학 치주과학교실) ;
  • 배기환 (충남대학교 약학대학) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 정종평 (서울대학교 치과대학 치주과학교실) ;
  • 최상묵 (서울대학교 치과대학 치주과학교실)
  • Published : 2000.03.30
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Abstract

The purpose of this study was to evaluate the antimicrobial activity of Crassirhzimae rhizoma and its possible use as an oral antiseptics for prevention of periodontitis. Its antibacterial activity against periodontopathic microorganisms including Actinobacillus actiomycetem - comitans, Capnocytophaga ochracea, Streptococcus mutans, Porphyromonas gingivalis, Prevotella intermedia, Actinomyces viscosus, Fusobacterium nucleatumwas evaluated via modified stab culture method. The cytotoxicity against gingival fibroblasts and rat osteoblasts was investigated via [$^3H$]thymidine incorporation and cellular activity was investigated via MTT assay. Chlorhexidine was used as control group. Crassirhizomae rhizoma was prepared at concentrations of 0.2, 0.15, 0.1, 0.05%. Chlorhexidine was also prepared at the same concentration. Crassirhizomae rhizoma showed lower antimicrobial antivity against these microorganism than chlorhexidine, but this difference was not significant. And, Crassirhzomae rhizoma showed more cellular activity and less cytotoxicity than chlorhexidine on human gingival fibrablast and rat osteoblast. This study suggests that Crassirhzomae rhizoma might be a candidate for a safe oral antiseptic for the prevention and treatment of periodontal disease.

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