식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

시클라멘 (Cyclamen persicum Mill.)의 조직배양에 의한 식물체 재분화

Plantlet Regeneration by Tissue Cultures of Cyclamen persicum Mill.

  • 은종선 (전북대학교 농과대학 생물산업연구소) ;
  • 김영선 (남도대학 원예산업과) ;
  • 한상권 (북면초등학교)
  • 발행 : 2000.11.01
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초록

시클라멘의 급속증식체계를 확립하기 위하여 발아 후 자엽이 전개된 어린 식물체의 괴경, 자엽, 엽병 등의 절편을 분리하여 절편부위별로 캘러스 및 기관분화에 미치는 생장조절제의 종류와 농도에 따른 식물체의 생장반응을 조사하였다. 절편부위별 캘러스 형성 및 shoot분화에 있어서 auxin류 단용처리의 경우 괴경절편의 NAA처리구에서 절편으로부터 캘러스 없이 shoot가 직접 발생하여 가장 양호하였으며, 1개의 절편체에서 10개 이상의 다아체가 분화되었다. 자엽이나 엽병절편은 캘러스만 유도되었고 대부분 치상당시의 상태로 shoot 발생에 auxin류의 처리는 부적합하였다. Cytokinin류 단용처리는 1.0 mg/L TDZ처리구에서 괴경의 경우 100％ shoot 분화율을 보였고, 자엽절편 역시 배양 40일경부터 다수의 shoot가 발생되어 양호하였다. NAA와 BA혼용처리는 0.5 mg/L NAA와 0.5 mg/L BA 혼용첨가된 배지에서 괴경. 자엽, 엽병절편 모두 shoot 분화가 양호하였으며, auxin류와 cytokinin 류 단용처리보다 NAA와 BA 혼용처리가 모들 절편에서 양호한 결과를 보였다. 뿌리발생을 유도하기 위해 NAA와 IBA 단용처리에 shoot를 배양한 결과 배양 15일경부터 0.5 mg/L IBA처리구에서 뿌리가 발생되기 시작하여 배양 30일 후에는 Pot이식이 가능할 정도로 뿌리가 발생되어 가장 양호한 결과를 보였다.

This study was conducted to examine the effects of explant sources and plant growth regulators on mass propagation of Cyclamen persicum. Tuber, cotyledon, and petiole tissues were cultured on MS medium supplemented with different concentrations and combinations of auxins and cytokinins. Shoots were not induced from calli on cotyledon and petiole explants cultured on MS medium containing various concentrations of 2,4-D or NAA. However, multiple shoots were formed directly from tuber explants cultured on the medium containing 0.5 and 1.0 mg/L 2,4-D or NAA. In MS medium with cytokinin alone, TDZ was more effective in shoot formation than BA or kinetin in all explants. The combinations of NAA and BA was found to be most effective in shoot formation from tuber, cotyledon and petiole explants. Especially, shoots were formed from all the tuber explants on the medium containing 0.5 mg/L of NAA and BA. Hormonal effects on root formation were examined by subculturing single shoots on MS medium containing NAA or IBA. The medium with 0.5 mg/L IBA was most effective in root induction and subsequent plantlet regeneration.

키워드

참고문헌

  1. Korean J. Plant Tissue Cult v.22 no.4 Plantlet regeneration by cotyledon and petiole cultures of Cyclamen persicum Mill Eun JS;Ko JA;Kim YS
  2. PHM Revue Horticole v.224 Premiere etape vers unemultiplication vegetative industrielle du Cyclamen par laculture in vitro Fersing JA;Mouras A;Lutz A
  3. Acta Hort. v.78 Morphogenesis and plant regeneration from cultured organ fragments of Cyclamen perslcum Geier T
  4. Plant Cell, Tissue ε Organ Culture v.28 The effect of explant material on somatic embryogenesis of Cyclamen persicum Mill Kiviharju E;Tuominen U;Tormala T
  5. Physiol. Plant. v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Marashige T;Skoog F
  6. Planta v.47 Wachstum und organbildung an in vitro kultivierten segmenten von Pelargonium zonale und Cyclamen persicum Mayer L
  7. Maraichers v.158 La multiplication vegetative du cyclamen a partir depetiole foliaire pemettra-telle une nouvelle application de la culture "in virto" horticulture. Pepiieristes; Horticulteurs Morel G
  8. Acta Hort v.226 Improvement on clonal propagation of cyclamen in vitro by the use of etiolated petioles Murasaki K;Tsurushima H
  9. J. Japan Soc. Hort. Sci. v.49 Vegetative propagation of cyclamen by notching of tuber. Ⅱ. Effect of scooping site and notching size on the regeneration of cyclamen tuber Nakayama M
  10. J. Japan Soc. Hort. Sci v.38 Aseptic culture of cyclamen tuber tissue Okumoto H;Takabayashi S
  11. International Symposium on Propagation of Ornamental Plant In Vitro propagation of Cyclamen persicum Mill Schwenkel HG;Grunewaldt J
  12. Acta Hort v.226 In vitro propagation of Cyclamen persicum Mill Schwenkel HG;Grunewaldt J
  13. Planta v.53 Gleichzeitige Induktion von Sprossen und Wuzeln an in vitro Kultivierten Gewebestucken von Cyclamen persicum Stichel E
  14. Plant Cell. Tissue ε Organ Culture v.34 In vitro regeneration through organogenesis of Meconopsis simplicifolia- an endangered omamental species Sulaiman IM;Babu CR