Journal of Microbiology and Biotechnology
- Volume 9 Issue 1
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- Pages.113-118
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- 1999
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Identification of an Embryonic Growth Factor IGF-II from the Central Nervous System of the Teleost, Flounder, and Its Expressions in Adult Tissues
- Kim, Dong-Soo (Department of Microbiology, Pukyong National University) ;
- Kim, Young-Tae (Department of Microbiology, Pukyong National University)
- Published : 1999.02.01
Abstract
The insulin-like growth factor (IGF) is found in all vertebrates and its type-II molecule is regarded as a fundamental embryonic growth factor during development. We have firstly identified, in this study, a cDNA clone corresponding to IGF-II (flIGF-II) from the adult brain of the teleost, Paralichthys olivaceus. We also examined the tissue expression of flIGF-II in several adult tissues by RT-PCR. The flIGF-II cDNA contained a complete ORF consisting of 215 amino acids and one stop codon. Its molecular characteristics appear to be similar to the previously identified IGF-II molecules, in which a common primary structure exhibiting B, C, A, D, and E domains is evidently observed. This cDNA clone seems to be cleaved at
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