글라디올러스 'Topaz' 캘러스의 기관형성에 미치는 배양 조건의 영향

Effects of Culture Conditions on Organogenesis in Gladiolus 'Topaz' Callus

  • 최정두 (영남대학교 자연자원대학 원예학과) ;
  • 변미순 (영남대학교 자연자원대학 원예학과) ;
  • 김규원 (영남대학교 자연자원대학 원예학과)
  • 발행 : 1999.10.01

초록

본 연구는 글라디올러스 캘러스로부터의 재분화 조건을 구명함으로써 캘러스 배양시스템을 확립하는데 기여하고자 하였다. 글라디올러스 캘러스로부터의 유식물체 획득은 캘러스를 2,4-D 무첨가 1/2 MS고체배지에 치상한 후 24시간 일장하에서 15$^{\circ}C$로 배양하였을 때 효과적이었다. 캘러스로부터의 기관형성에 미치는 배양방법의 영향을 검토한 결과, 부정근의 형성은 액체진탕배양이 그리고 부정아의 형성은 액채정치배양이 각각 우수한 것으로 확인되었다. 본 실험의 결과를 통해 글라디올러스 ‘Topaz' 캘러스로부터의 재분화를 위한 최적 배양 조건을 선발하는 한편, 액체진탕배양법에 의해 기관-캘러스 혼합체를 유도할 수 있었으며, 이를 이용하여 기내 유식물체를 대량생산할 수 있는 가능성을 확인할 수 있었다.

This study was carried out to establish improved techniques on organogenesis from callus culture of Gladiolus. Organogenesis from the callus was effective in the half strength of MS solid medium without 2,4-D at 15 $^{\circ}C$ under 24 hours of daylength. Formation of adventitious root was most effective in the liquid shaking culture, and adventitious shoot induction was effective in the liquid stationary culture. From these results, we could find optimal culture conditions for redifferentiation from callus, in addition, liquid shaking culture revealed as more useful when compared with that of solid culture method for the redifferentiation of callus in Gladiolus `Topaz'.

키워드

참고문헌

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