식물조직배양학회지 (Korean Journal of Plant Tissue Culture)

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

낙엽송 (Larix leptolepis) 배발생조직의 초저온보존 및 식물체 재분화

Cryopreservation of Embryogenic Tissue and Plant Regeneration in Larix leptolepis

  • 김용욱 (임업연구원 임목육종부) ;
  • 김준철 (강원대학교 생물학과) ;
  • 윤양 (임업연구원 임목육종부) ;
  • 노의래 (임업연구원 임목육종부) ;
  • 손성호 (임업연구원 임목육종부)
  • Kim, Yong-Wook (Department of Tree breeding, Forestry Research Institute) ;
  • Kim, Joon-Chul (Department of Biology, Kangwon National University) ;
  • Youn, Yang (Department of Tree breeding, Forestry Research Institute) ;
  • Noh, Eu-Rae (Department of Tree breeding, Forestry Research Institute) ;
  • Son, Sung-Ho (Department of Tree breeding, Forestry Research Institute)
  • 발행 : 1999.10.01
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초록

본 연구에서는 낙엽송 배발생조직의 장기저장을 위한 가능성에 대하여 조사하였다. 0.4M혹은 20% PEG로 24시간 전 처리하여 -0.33$^{\circ}C$/min의 동결온도 하강율로 초저온시켰을 때 높은 조직생중량을 보인 반면 -0.5 혹은 -1.$0^{\circ}C$/min의 경우 초저온 후 조직의 생중량이 저조한 것으로 나타났다. 1, 7, 및 28일간 초저온보존된 조직을 회수하여 재생장시킨 다음 PCR을 이용하여 변이분석을 한 결과 초저온보존 기간에 관계없이 DNA변이는 전혀 나타나지 않았다. 초저온보존된 배발생 조직으로부터 체세포배 유도 및 식물체 재분화를 유도할 수 `있었다.

The possibility for long-term preservation of Larix leptolepis embryogenic tissue was tested in this study. Higher relative increase of the tissue fresh weight was observed when embryogenic tissue was pretreated for 24 hrs in a medium containing 0.4 M sorbitol or 20% polyethyleneglycol with cooling rate of -0.33$^{\circ}C$/min. The fast cooling rate of -0.5$^{\circ}C$ and -1.$0^{\circ}C$/min appeared to be less effective in regrowth of tissues from cryopreservation. No DNA variants have been observed by PCR analysis among the embryogenic tissues recovered after 1-, 7-, and 28-day-cryopreservation. The post-thaw embryogenic tissue gave rise to mature somatic embryos which developed into plants.

키워드

참고문헌

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