Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Organogenesis and Plant Regeneration in Tissue Cultures of Lettuce Seedling Explants

상추 유식물체 절편의 조직배양에 의한 고빈도 기관발생과 식물체 재분화

  • Jung, Min (Plant Cell and Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Woo, Je-Wook (Plant Cell and Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung, Won-Joong (Plant Cell and Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoo, Jang-Ryul (Plant Cell and Molecular Biology Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • 정민 (생명공학연구소 식물세포 및 분자생물학 R.U.) ;
  • 우제욱 (생명공학연구소 식물세포 및 분자생물학 R.U.) ;
  • 정원중 (생명공학연구소 식물세포 및 분자생물학 R.U.) ;
  • 유장렬 (생명공학연구소 식물세포 및 분자생물학R.U.)
  • Published : 1999.07.01
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Abstract

To induce adventitious buds, hypocotyl and cotyledonary explants from 7 to 10 day-old seedlings of lettuce (Lactuca sativa L.: two Japanese cultivars of crisphead lettuce and four Korean cultivars of leaf lettuce) were cultured or Murashige and Skoog (MS) and Schenk and Hildebrandt (SH) media supplemented with BA and NAA in the light for five weeks. Cotyledonary explants produced adventitious shoots at greater frequencies than hypocotyl explants. MS medium was more favorable to adventitious shoot formation than HS medium. Combination of 0.5 mg/L BA and 0.1 to 1 mg/L HPh in MS medium led to the greatest frequency (86%) in adventitious shoot formation. Creator than 95% of shoots excised from explants were rooted when cultured on MS basal medium.

부정아를 유도하기 위하여 발아 7∼10일된 일본 결구상추 2품종과 국내 잎상추 (Lactuca sativa L.)4품종의 하배축 및 자엽절편으로부터 식물체 재분화시스템을 확립하였다. 하배축 및 자엽절편을 BA와 NAA가 조합 처리된 MS 및 SH배지에 치상하여 5주 동안 명배양하였다. 일반적으로 하배축보다는 자엽절편에서, SH 배지보다는 MS 배지에서 배양하였을 때에 부정아 형성률이 높았다. MS배지에 BA는 0.5 ㎎/L, NAA는 0.1∼l ㎎/L로 처리하였을 때에 최고의 부정아 형성률을 보였으며, 품종에 따라 30% ( '만추대청치마')에서 86% 까지의 부정아 형성률을 나타내었다. 형성된 shoot의 95% 이상이 MS 기본배지에서 발근하여 소식물체로 발달하였다.

Keywords

References

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