Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Low Temperature Pretreatment on Pollen Dimorphism and Embryo Formation in Anther Culture of Platycodon grandiflorum

도라지 (Platycodon grandiflorum) 약배양에서 저온처리가 화분 2형현상 및 배형성에 미치는 영향

  • 고정애 (전북대학교 원예학과, 농업과학기술연구소)
  • Published : 1999.07.01
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Abstract

In order to investigate the effect of low temperature pretreatment on pollen dimorphism and embryo formation in anther culture of Platycodon grandiflorum, the anthers with microspore at the uninucleate stage were cultured on Murashige and Skoog medium supplemented with 0.5mg/L NAA and 1.0 mg/L BA. The low temperature pretreatment have clear effect on the frequencies of S pollen grains, symmetrical binucleate microspores (B type of S pollen), multinucleate and multicelled pollen grains. Especially, after low temperature pretreatment at 8$^{\circ}C$ for 5 days increased the frequency of S pollen grain (20.6%) in vivo. In addition, the highest frequency of callus induction (54.9%) and embryo formation (9.9%) were obtained from the anther pretreatment at 8$^{\circ}C$ or 5 days. Three distinct pathways could be recognized in the androgenesis, one involving mainly the vegetative cell, the second starting with the vegetative and the generative cell, respectively, and the third accompaning with two equal vegetative type cells in the pollen grains.

도라지 약배양에 있어서 저온처리가 화분 2형현상과 배형성에 미치는 영향을 조사하기 위해 1핵성 소포자기의 도라지 약을 0.5 mg/L NAA와 1.0mg/L BA가 첨가된 MS배지에 배양하였다. 저온전처리는 이상화분과 균등분열에 의한 B형 화분, 다핵 및 다세포 화분수를 현저하게 증가시켰는데 특히 8$^{\circ}C$에서 5일간 저온전처리는 배양 전 이상화분이 20.6%가 증가되었고 배양 중에는 54.9%의 캘러스와 9.9%의 배형성율을 나타내 가장 효과적이었다. 배양 중 소포자로부터 배는 첫째. 주로 영양세포가 분열하거나 둘째, 영양세포와 생식세포가 각각 분열하거나 셋째, 균등분열에 의한 영양핵과 동일한 크기의 두 낭세포 모두가 분열하여 배를 형성하는 3가지 경로가 관찰되었다.

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References

  1. Crop. Improv v.4 In vitro induction of haploids in wheat (Triticum aestivum L.) Bajaj, Y. P. S.
  2. Planta(Berl.) v.124 Pollen dimorphism and anther culture in barley Dale, P. J.
  3. Kor. J. Plant. tiss. Cult. v.12 Anther culture and pollen dimorphism Harn, C. Y.
  4. Planta(Berl.) v.147 The frequency of embryogenic pollen grains is not increased by in vitro anther culture in Nicotiana tabacum L. Horner, M.;Mott, R. L.
  5. Ann. Bot. v.42 Pollen dimorphism-origin and significance in pollen plant formation by anther culture Horner, M.;Street, H. E.
  6. Ph. D. thesis Studies on the pollen dimorphism an danther culture of Anemone coronaria Ko, J. A.
  7. J. Kor. Soc. Hort. Sci. v.37 Embryogenesis and plant regeneratio by the anther culture of Zantedeschia aethiopica spp Jo, J. A.;Kim, Y. S.;Eun, J. S.
  8. Heredity v.3 Nuclear differentiation in the pollen grain La Cour, L. F.
  9. Kor.J. Plant. Tiss. Cult. v.20 Embryogenesis and plant regeneration in anther cultures of Platycodon grandiflorum Lee, B. K.;Ho, J. A.l;Kim, Y. S.;Eun, J. S.
  10. Kor. J. Plant. Tiss. Cult. v.16 Eggects of pollen fimorphism and plant growth regulators in anther culture of Paeonia albiflora Lee, B. K.;Eun, J. S.;Ko, J. A.;Kang, N. J.
  11. Physiol. Plant. v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige, T.;Skoog, F.
  12. C. R. Hebd Senac Acad Sci, Paris v.276 Effet d' un choc thermique sur le pouvori embryogene du pollen de Datura innoxia cultive dans l' antherou isole de ;' anthere Nitsch, C.;B. Norrell
  13. Amer. J. Bot. v.65 Origin and denelopment of pollen embryoids and pollen calluses in cultured anther segments of Hyoscyamus niger (henbane) Raghavan, V.
  14. Euphytica v.23 The origin of unreduced microspores due to aberrant cytokinesiss in the meiocytes of potato and its genetic significance Ramana, M. S.
  15. Plant. Cell. Tiss. Org. Cult. v.6 Anther culture of Sorghum bicolor (L.) Moench. Rose, J. B.;Dunwell, J. M.;Sunderland, N.
  16. Haploids in Higher Plants.Advances and Potential Abther culture as a means of haploid induction Sunderland, N.;Kasha, K. J.(ed.)
  17. Proceeding of symposium in plant tissue culture Strategies in the improvement of yields in anther culture. Sunderland, N.
  18. Plant Tissue and Cell Culture. Street HE (2nd eds.) Anther and pollen culture Sunderland, N.;Dunwell, J. M.
  19. Exp. Bot. v.22 Embryoid formation in pollen grains of Nicotiana tabacum Sunderland, N.;Wick, F. M.