Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of Ascorbate on Somatic Embryogenesis in Carrot Cell Cultures

당근 세포배양으로부터 체세포배 발생에 미치는 아스콜빈산의 효과

  • 소웅영 (전북대학교 자연과학대학 생물과학부, 전북대학교 기초과학연구소) ;
  • 김이엽 (전북대학교 자연과학대학 생물과학부, 전북대학교 기초과학연구소) ;
  • 조덕이 (우석대학교 생물학과)
  • Published : 1999.07.01
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Abstract

This study was conducted to elucidate the effects of ascorbic acid and dehydroascorbic acid on somatic embryogenesis from the cultured cells of carrot. Ascorbic acid in culture medium merely stimulated the proliferation of non-embryogenic cells but dehydroascorbic acid in medium induced embryogenic cells from non-embryogenic cells accompanying the inhibition of cell proliferation. Ascorbic acid in medium inhibited somatic embryogenesis from embryogenic cells while dehydroascorbic acid in medium enhanced somatic embryogenesis from the cells as well as non-embryogenic cells. This enhancement was limited to globular embryos and the maturation to cotyledonary embryos was inhibited by dehydroascorbic acid treatment. From the above results it is suggested that carrot callus cultures on medium containing dehydroascorbic acid could quickly induce embryogenic cells. In addition after brief culture of embryogenic cells on development medium containing dehydroascorbic there by acid the subculture of the cells to MS basal medium resulted in the high frequency production of somatic embryos.

당근의 배양세포로부터 체세포배의 발생과정에 미치는 아스콜빈산 및 dehydroascorbic acid의 영향을 밝히기 위하여 본 실험을 시도하였다. 비배발생세포의 배양에 처리된 아스콜빈산은 세포증식을 촉진시켰을 뿐인데 dehydroascorbic acid는 세포증식을 억제시키면서 배발생세포로 전환시킨 효과가 있었다. 배발생세포의 배양에 처리된 아스콜빈산은 체세포배 발생을 억제시켰지만 dehydroascorbic acid는 체세포배발생을 촉진시켰다. 그러나 이와 같은 발생촉진은 구상배에서 중단되므로 성숙에는 오히려 저해적이었다. 이상의 결과로부터 당근의 캘러스배양에 dehydroascorbic acid를 처리하여 빠른 시일내에 배발생캘러스를 확보한 다음 dehydroascorbic acid 첨가 배발생 배지에서 초기배발생기간 배양 후 MS 기본배지로 옮겨 배양하면 고빈도의 체세포배생산 실험계가 확립될 것으로 판단된다.

Keywords

References

  1. Caryologia v.42 Ascorbic acid effect on pericycle cell line in Allium ceparoot Arrigoni, O.;Bitonti, MB.;Cozza R.;Innocenti, AM.;Liso, r.;Veltri, R.
  2. Kor. J. Plant. Tiss. Cult. v.21 Somatic embryogenesis in cultures of Korean soybean (Glycine max L.) culrivars an effects of 2, 4-dichlorophenoxyacetic acid concentratio on somatic emvryo morphology Choi, P. S.;Soh, W.-Y.;Cho, D. Y.;Liu, J. R.
  3. Physiol Plant v.92 Ascorbic acid effect on the onset of cell priferation in pea root Citterio S;Sgorbati S;Scippa S;Sparvoil E
  4. J. Plant. Groeth Reg. v.15 Rooting hastened in onions by ascorbate an dascoebate free rasical Cabo, R. C.;Goncalez-Reyes, J. A.;Cordoba, F.;Navas, P.
  5. Plant Phusiol v.85 Control of wild carrot somatic embryo development by antioxidants Earnshaw, B. A.;Fohnson, M. A.
  6. New Phytol. v.114 The size of quiescent center in roots of Allium cepa L. grown ascorbic acid Innocenti, A. M.;Bitonti, M. B.;Arrigoni, O.;Liso, R.
  7. Plant. Cell. Tissl Org. Cult. v.13 Ascorbic acid enfancemene of organogenesis in tobacco callus Joy IV R. W.;Patel, K. R.;Thorpe, T. A.
  8. Thumb.Kor. J. Plant. Tiss. Cult. v.20 Somatic embryogenesis an dstructural aberrancy of embryos in tissue culturesj of Aralia cordata Lee, K. S.;Soh, W. Y.
  9. Exp. Cell. Res. v.150 Relationship between ascorbic acid and cell division Liso, R.;Calabrese, G.;Bitonti, M. B.;Attigone, O.
  10. New phytol. v.110 Ascorbic asid induced progression of quiesenct center cells from G1 to Sphase Liso R.;Innocenti, A. M.;Bitonti, M. B.;Arrgoni, O.
  11. Physiol Plant. v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige, T.;Skoog, F.
  12. Plant. Cell. Tiss. Org. Cult. v.19 Effects of ascorbic acid on axillary shoot induction in Tylophora indica (Burm. f) Merril Sharma, N.;Chandel K. P. S.
  13. Plant. Cell. Rep. v.12 Plant regeneratio in a jute species (C. caspularis) and its possible relationship with glyoxalase-1. Seraj, Z. I.;Sarker, A. B.;Islam, A. S.
  14. Proc. (7th) Symp. Plant. Biotech. Bot. Soc. Korea Developmental and structural diversity of regenerated plants in cell and tissue cultures. Mol Apro Plant Cell Differ Soh, W. Y.
  15. Proc. (2nd) Asia-Pacific Conf. Plant. Cell. Tiss. Cult. Beijing Germinability and Cotyledon structure of somatic embryos in some dicotylesonous plant Soh, W. Y.
  16. J. Plant. Biol. v.39 Multicotyledonary structure of somatic embryos formed from cell cultures of Daucus carota L. Soh, W. Y.;Cho, D. Y.;Lee, E. Y.
  17. Plant. Cell. Rep;. v.10 The role of ethylene and reducing agents on ahther culture of tetrapliod potato (Solamum tuberosum L.) Tiainen, T.
  18. Plant. Sci. v.92 The relationship between somatic embryo morphology an conversion in peanut (Arachis hypogaea L.) Wetzstein, H. Y.;Baker, C. M.