Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Determination of p-Methoxycinnamic Acid in Scrophulariae Radix by High Performance Liquid Chromatography

고속액체크로마토그라피에 의한 현삼근(玄蔘根) 중 p-Methoxycinnamic acid의 정량

  • Lee, Sang-Hyun (Natural Products Research Institute, Seoul National University) ;
  • Chi, Hyung-Joon (Natural Products Research Institute, Seoul National University) ;
  • Shin, Kuk-Hyun (Natural Products Research Institute, Seoul National University)
  • 이상현 (서울대학교 천연물과학연구소) ;
  • 지형준 (서울대학교 천연물과학연구소) ;
  • 신국현 (서울대학교 천연물과학연구소)
  • Published : 1999.09.30
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Abstract

A new method for quantitative determination of p-methoxycinnamic acid in Scrophulariae radix by high performance liquid chromatography was established. A reversed-phase system with SPHERI-5 RP-18 5 micron $(250\;{\times}\;4.6\;mm)$ column using TFA (0.05%) and acetonitrile as a mobile phase was developed. p-Methoxycinnamic acid and N-(p-methoxycinnamoyl)-p-aminophenol as an internal reference were detected at 310 nm and the analysis was successfully carried out within 50 min.

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