원예과학기술지 (Horticultural Science & Technology)
- 제16권4호
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- Pages.508-510
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- 1998
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- 1226-8763(pISSN)
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- 2465-8588(eISSN)
감자 유전자원의 기내 장기보존 방법
In vitro Long Term Conservation of Potato Germplasms
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Yi, Jung-Yoon
(National Alpine Agricultural Experiment Station, RDA) ;
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Cho, Hyun-Mook
(National Alpine Agricultural Experiment Station, RDA) ;
- Park, Kuen-Woo (Dept. of Horticultural Science, Korea University)
- 발행 : 1998.12.31
초록
감자 유전자원의 기내 유식물체 장기보존시 식물체 변이발생을 최소화하면서 최장기간 배양할 수 있는 배양조건을 확립하기 위해 생장억제물질을 사용하지 않고 탄소공급원으로서 삼투조절제를 sucrose와 혼합처리하였는데, 이러한 삼투조절제에 의한 생육지연 효과는 배양온도를 낮춤으로써 효과를 증대시킬 수 있었다. 6개월 저장 후 저온하의 4배체 계통의 초장이 2배체 계통의 초장보다 다소 높게 나타났으나 배지간 차이는 나타나지 않았다. 그러나 저온저장 12개월 후에는 mannitol과 sucrose의 혼합처리인 LSM 2 배지보다 sorbitol과 sucrose의 혼합처리인 LSM 1 배지의 처리계통에서 만족할 만한 결과를 얻을 수 있었으며, 특히 2배체 근연야생종인 stn-16은 LSM 1 배지에서 75%의 상당히 높은 생존율을 보였으며 재배종인 'Atlantic'은 두 배지 모두에서 100%의 생존율을 보였다.
This study was carried out to establish the in vitro culture system of potato germplasms for minimizing the occurrence of variation and maximizing the culture period. We used osmoticum such as sorbitol or mannitol with sucrose in the absence of plant growth regulators. The growth of potato germplasms in the medium containing osmoticum was increased when the growth temperature was lowered. After six months storage in low temperature, plant heights of tetraploid was somewhat higher than those of diploid with the exception of stn-16 and the difference due to media was not observed. But after twelve months storage, survival rates of plants cultured in LSM 1(sucrose and sorbitol) was higher than those of plants cultured in LSM 2(sucrose and mannitol). The survival rate of stn-16, diploid wild species, was approximately 75% and it was considerably high. In Atlantic, tetraploid cultivated variety, every individual was survived.