The Inhibitory Effects of Taraxaci Herba against Cadmium induced Cytotoxicity

포공령의 카드뮴에 대한 세포독성 억제효과

  • Han, Du-Seok (Department of Oral Anatomy, School of Dentistry) ;
  • Lee, Ki-Nam (Department of Preventive Medicine, School of Oriental Medicine) ;
  • Lee, Jong-Sub (Department of Preventive Medicine, School of Medicine) ;
  • Baek, Seung-Hwa (Department of Chemistry, College of Natural Sciences, Wonkwang University)
  • 한두석 (원광대학교 치과대학 구강해부학교실) ;
  • 이기남 (한의과대학 예방의학교실) ;
  • 이종섭 (의과대학 예방의학교실) ;
  • 백승화 (자연과학대학 화학과)
  • Published : 1998.06.01

Abstract

This study was carried out to evaluate antitoxic effects Taraxaci Herba extract against Cadium by calorimetric methods. The antitoxic activity of Taraxaci Herba ex tract in NIH 3T3 fibroblasts was evaluated by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-phenyl-2H-tetrazoliumbromide), NR (Neutral red) and SRB (Sulforhodamine B protein) assay. The light microscopic study was carried out to observe morphological changes of the treated cells. These results were obtained as follows; The concentration of $10^{-2}mg/ml$ of Taraxaci Herba extract was shown significant antitoxic activity. The number of NIH 3T3 fibroblasts were antitoxic and tend to regenerate. These results suggest that Taraxaci Herba extract retains a potential antitoxic activity.

Keywords

References

  1. 대한한의학회지 v.13 포공령 수추출물이 진통, 항염작용에 미치는 영향 김석근;송호준
  2. 한국생약회지 v.24 포공령의 항위염 작용 이은방;김정근;김옥경
  3. 원광대학교 석사학위논문 이명호
  4. 대한화학회 한국산 생약으로 부터 항암물질의 개발 (제 8보). 비색분석법에 의한 포공령 물추출물의 항암평가 오인교;유은하;한두석;백승화
  5. Toxicol. Appl. Pharmacol. v.74 Transient induction of hepatic metallothionein following oral ethanol administration Waalkes, M. P.;Hjelle, J. J.;Klaassen, C. D.
  6. Biological stains Conn, H. J.
  7. 한국생약 학회지 v.25 소엽의 세포 독성 및 항암작용에 관한 연구 한두석;정병호;유현경;김영옥;백승화
  8. J. Immunol. Methods v.65 Rapid colorimetric assays for cellular growth and survival: application to proliferation and cytotoxicity assays Mosmann, T.
  9. Tissue Culture Meth. v.9 A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/Nr-90) Borenfreund, E.;Puerner, J. A.
  10. J. Natl. Cancer Inst. v.82 New colorimetric cytotoxicity Assay for Anticancer-drug screening Skehan, P.;Storeng, S.;Studiero, D.;Monke, A.;McMahon, J.;Vistica, D.;Warren, J. T.;Bodesh, H.;Kenny, S.;Boyd, M. R.