분석과학 (Analytical Science and Technology)
- 제10권5호
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- Pages.325-331
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- 1997
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- 1225-0163(pISSN)
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- 2288-8985(eISSN)
Modified Micellar Electrokinetic Capillary Chromatography에 의한 폭약 성분의 분리능 향상
Improved Separation of Organic Explosives by Modified Micellar Electrokinetic Capillary Chromatography
- Park, Sung-Woo (Department of Forensic Science, National Institute of Scientific Investigation) ;
- Yang, Young-Geun (Department of Forensic Science, National Institute of Scientific Investigation) ;
- Hong, Sungwook (Department of Forensic Science, National Institute of Scientific Investigation) ;
- Kim, Taek-Jae (Department of Chemistry, Kyonggi University)
- 투고 : 1997.03.31
- 발행 : 1997.10.25
초록
계면활성제로 sodium dodecylsulfate(SDS)를 이용한 micellar electrokinetic capillary chromatography (MECC) 방법을 이용하여 유기 폭약 성분을 신속하고 정확하게 분석할 수 있는 방법을 연구하였다. 완충요액으로는 2.5mM borate와 25mM SDS(pH 8.5)를 사용하였고, 완충용액에 1M urea와 10% 유기 용매(acetonitrile, methanol 및 ethanol)를 첨가한 결과 분리능이 향상되는 현상을 관찰할 수 있다. 15종의 폭약 혹은 그 구성 성분을 MECC 방법으로 분리한 결과 RDX/TNB 및 DNN/DEP를 제외한 성분을 분리할 수 있었고, 검출한계는 1~4ppm으로 나타났다.
Among various CE separation methods, micellar electrokinetic capillary chromatography(MECC) method using sodium dodecylsulfate(SDS) provides rapid and accurate separation of organic explosive constituents with easy. The running buffer was composed with 2.5 mM borate and 25mM SDS(pH 8.5). Addition of 1M urea and 10% organic modifiers (acetonitrile, methanol and ethanol) improves the resolution of adjacent explosive constituents. When 15 explosive constituents were developed in MECC, most constituents were separated successively while RDX/TNB and DNN/DEP were not, and detection limits of separated compounds are in range of 1 to 4 ppm.
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