Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Effect of Methionine Levels on Brain Lipid Peroxidation in Ethanol-treated Rats of Selenium Deficiency

메티오닌과 셀렌이 에탄올 중독된 흰쥐의 뇌지질과산화에 미치는 영향

  • 조수열 (영남대학교 식품영양학과) ;
  • 이미경 (영남대학교 식품영양학과) ;
  • 박은미 (영남대학교 식품영양학과) ;
  • 장주연 (영남대학교 식품영양학과) ;
  • 김명주 (대구산업전문대학 식품영양과)
  • Published : 1997.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was designed to investigate the effects of methionine(Met) on the activities of brain lipid peroxidation related enzymes in ethanol administrated rats of selenium(Se) deficiency. Male Sprague-Dawley rats were fed Se deficiency diets containing one of the three levels of Met (0, 3, 9g/kg diet) and ethanol(2.5g/kg of body weight) was administrated as 25v/v% ethanol treated groups orally. The rats sacrificed after 5 and 10 weeks of feeding periods. Alcohol dehydrogenase activity was increased in ethanol treated groups and was higher Met normal group than Met deficiency and excessive groups at 5 and 10 weeks dieting. Aldehyde dehydrogenase activity was decreased in ethanol treated groups and significantly decreased in Met deficiency group. Monoamine oxidase activity in brain was increased in ethanol treated groups and was predominently increased in Met deficiency groups. Superoxide dismutase and glutathione peroxidase activities were decreased in ethanol treated groups and tended to increase in proportion to level of dietary methionine. Glutathione S-transferase and catalase activities and lipid peroxide content were increased by ethanol administration and were higher Met deficiency group than normal and excessive groups.

에탄을 투여로 인한 뇌조직의 지질과산화에 미치는 식이성 Met과 Se의 효과를 구명코자 Se을 결핍시킨 횐쥐를 대상으로 Met을 수준(0, 3, 9g/kg diet)별로 급여 하였으며, 실험기간은 5주와 10주로 사육하여 뇌조직의 알코을 대사효소와 유리기 제거 효소계의 활성을 관찰하였다. 알코을 대사효소인 ADH 활성은 5주와 10주군에서 에탄을 투여시 증가되었고 Met정상급여군이 결핍과 과량급여군에 비해 유의적인 증가를 나타내었다. AIDH 활성은 에탄을 투여로 유의하게 감소되었고 특히 Met와 Se 동시 결핍시 감소가 가장 현저하였다. 투여기간이 길수록 활성은 감소하는 경향이었다. MAO활성은 에탄올 투여군이 대조군에 비해 유의적으로 증가하였으며, Met급여에 의해 활성은 억제되는 것으로 나타났다. SOD 활성은 에탄올 투여로 유의적인 감소를 보였으며 5주 및 10주 모두 Met 과량급여군이 결핍과 정상급여군에 비해 유의적으로 증가하였다. GSH-Px 활성은 에탄을 투여로 유의적으로 감소되 었으며, Met과 Se 동시 결핍군에서 가장 감소정도가 큰 것으로 나타났다. Met 급여수준이 증가할수록 그 활성은 유의적으로 증가하였으며, 10주군이 5주군에 비해 활성이 증가하였다. 에탄올 투여로 증가된 GST 활성은 Met결핍에 의해 더욱 가중되었으며 10주군이 5주군에 비해 GST 활성이 유의적으로 증가된 것으로 보아 장기간의 Met 결핍은 에탄을 중독을 심화시킬 것으로 생각된다. Catalase 활성은 에탄을 투여로 유의적인 증가를 나타내었고 Met 결핍군이 정상급여군 및 과량 급여군에 비하여 유의적인 증가를 보였다. GSH함량은 에탄올에 의해 유의적으로 감소되었는데 Met 급여 수준이 증가할수록 에탄올로 인해 감소된 GSH함량이 유의적으로 증가되었으며, 10주군이 5주군에 비해 증가 정도가 현저하였다. 과산화의 지표인 LPO 함량은 에탄을 투여시 증가되었고 Met 결핍군은 정상급여군에 비해 유의적으로 증가되었으며, 특히 Met 과량급여시 5주군에 비해 10주군이 유의적으로 증가하였다. 이 상의 결과에서 Se 결핍시 Met 급여로 인해 에탄올 대사효소의 활성 증가 및 에탄올에 의해 증가된 뇌의 지질과산화를 줄이는 것으로 보아 Met이 생체의 노화를 방지할 수 있을 것으로 생각되며, Se 결핍으로 증가된 지질과산화 반응에 대한 보호와 적응 수단으로써 방어 효소의 활성도가 높아진 것으로 사료된다.

Keywords

References

  1. Neurotoxicology v.15 Nutrition and alchol neurotoxicity Manzo, L.;Locatelli, C.;Candura, S.M.;Costa, L.G.
  2. Alcohol alcoholism v.25 Ethanol-in-duced lipid peroxidation and oxidative stress in extrahepatic in brain Tabakoff, B.;Gelpke, C. C.
  3. Adv. Exp. Med. Biol. v.56 Alcohol and aldehyde metabolim in brain Tabokoff, B.;Gelpke, C.C.
  4. J. Lab. Clin. Med. v.98 Ethanol induced lipid peroxidation : potentiation by chronic alcohol feeding and attenuation by methionine Shaw, S.;Jayatilleke, R.;Ross, W. A.;Gordon, E. R.;Lieber, C. S.
  5. J. Hepatology v.14 Sselenium deficiency and transsulfuration in the chick Halpin, K. M.;Baker, D. R.
  6. J. Nutr. v.114 Selenium deficiency and transsulfuration in the chick Halpin, K. M.;Baker, D. R.
  7. J. Nutr. v.107 Protective againtst carbon tetracholoride-induced lipid peroxidation in the rat by dietary vitamin E, selenium and methionine as measured by ethane evolution Hafeman, D. G.;Hoekstra, W. G.
  8. J. Nutr. v.107 Ad Hoccommittee on standards for nutritional atudies Report of the American Institute of Nutrition
  9. Biochem. Pharmacol. v.24 Effects of ethanol on hepatic microsomal drug-metabolizing enzymes in the rats Liu, S. J.;Ramsey, R. K.;Fallon, H. J.
  10. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry, O. H.;Rosebrough, N. J.;Farr, A. L.;Randall, R. J.
  11. Methods of enzymatic analysis Bergmayer, H. U.
  12. Biochem. Biophs. Acta v.397 Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution Koivula, T.;Koivusalo, M.
  13. Proc. Natl. Aca. Sci. v.84 Corelation of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrine neurotoxicity anion radical in the autooxidatin of pyrogallol&a convenient assay for superoxide dismutase Marklund, S.;Marklund, G.
  14. Eur. J. Biochem. v.47 Involvement of the superoxide anion radical in the autooxidation of pyrogallol&a convenient assay for superoxide dismutase Marklund, S.;Marklund, G.
  15. J .Lab. Clin. Med. v.70 Studies on the quantitative and qualitative charateization of erythrocytes glutathione peroxidase Paglia, E. D.;Valentine, W.N.
  16. J. Biol. Chem. v.249 Glutathione S-Tranferase : The first enzymatic step in mercapturic acid formation Habig, W. H.;Pabist, M. J.;Jakoby, W. B.
  17. Methods of enzymetic analysis v.2 Catalase Aebi, H;H. U.(ed.)
  18. Arch Biochem. Biophy. v.82 Tissue sulfhydryl group Ellman, G. L.
  19. Anal. Biochem. v.95 Assay for lipid peroxides in animal tissure by thiobarbituric acid reaction Ohkawa, H.;Ohishi, N.;Yaki, K.
  20. Statistical methods. 6th. Senedecor, G. W.;Cochrane, W. G.
  21. Adv. Exp. Med. Biol. v.56 Effects of ethanol on brain metabolism Rawat, A. K.
  22. Canad. J. Biochem. v.13 Effect of aliphatic alcohols and aldehydes on the metabolism of potassium-stimulated rat brain cortex slices Majchrowizz, E.
  23. Acta Chem. Scand. v.15 Effect of ethanol dehydrogenase II Theorell, H.;Kulonen, E.
  24. Life, Sci. v.7 The effect of ethanol and disulfiram on serotonin-C14 metabolism in rat liver hemogenates Tabakoff,B.;Williamson,O.
  25. J. Pharmacol Exp. Ther. v.165 Metabolism of some phenylethylamines and thier β-hydroxylated analogs in brain Breese, G. R.;Chase, T. N.;Kopin, I. J.
  26. J. Biol. Chem. Horse liver aldehyde dehydrogenase II. Kinetics and mechanistic implications of the dehydrogenase and esterase activity Feldman, R. I.;Weiner, H.
  27. Toxicology v.45 Enhancement by glutahione depletion of ethanol-induced acute hepatotocixity in vitro Strubelt, O;Younes, M.;Pentz, R.
  28. J. Nutr. v.104 Effect of dietary selenium and autoxidized lipis on the glutathione peroxidase system of gastrointestinal tract and other tissues in the rat Reddy, K.;Tappel,A.L.
  29. Biochem. J. v.248 The effect of selenium and copper deficients on glutathione S-transferase and glutathione peroxidse in the rat liver Arthure, J. A.;Morrice, P. C.;Nicol, F.;Beddows, S. E.;Boyd, R.;Hyes, J. D.;Beckett, G. J.
  30. Trans. Am. Soc. Neurochem. Abs. v.5 Monoamine-dependent ethanol oxidation in mouse brain Anderson, R. A.;Schulman, M. P.
  31. Arch. Biochem. Biophys. v.154 Role of $H_2O_2$ generation in perfused rat liver and the reaction of catalase-compound I and hydrogen donors Oshino, N.;Chance, B.;Sies, H.;Bucher, T.
  32. Free Radic. Biol. Med. v.7 Introduction : Role of lipid peroxidation and oxidative stress in alcohol toxicity Cederbaum, A. I.