한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제25권5호
  • /
  • Pages.490-494
  • /
  • 1997
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  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Enterobacter agglomerans TY-25 에 의한 D-Galactose로부터 D-Tagatose의 생산

  • 김상용 (동양제과(주) 기술개발연구소) ;
  • 노회진 (동양제과(주) 기술개발연구소) ;
  • 오덕근 (우석대학교 식품공학과)
  • 발행 : 1997.10.01
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초록

A variety of microbial strains isolated from soil were tested for their ability to produce D-tagatose from D-galactose. An organism that can convert D-galactose into D-tagatose was selected and was identified as Enterobacter agglomerans. The cells grown on the induction medium containing 20 g/l arabinose were found to the best conversion potential among different carbohydrates and the conversion yield was about 15% when 20 gll galactose was used. The isolated crystals were obtained from the culture broth after the purification process such as treatment of ion resins, crystallization, and drying. The recovery yield was 70% after the purification. The crystals were identified as D-tagatose by the infrared spectroscopy, HPLC, specific optical rotation, and melting point.

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참고문헌

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