• Korean Journal of Microbiology
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• v.26 no.1
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• pp.20-26
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• 1988

Three $NIf^{-}$ -mutants were isolated from Enterbacter agglomerans 339 through the transposon umtagenesis using a RP4-mobilising system for its nif-gene characterization. All mutants hadn't acetylene-reduction ability. Then we confirmed that Tn5 was inserted into all conserved nif-plasmids through the Southern Hybridization.

• Microbiology and Biotechnology Letters
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• v.15 no.2
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• pp.116-121
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• 1987

In order to cloning of the nif genes of Enterobacter agglomerans NFB-264, the digested total DNA of the strain was ligated to pBR 322 and transformed into E. coli. Through the negative selection and colony hybridization, the transformants were obtained. The recombinant plasmids, pNEL 10 and pNES 20 were extracted from these transformants. It was known from Southern hybridization that pNEL 10 contained the 12 Mdal foreign DNA fragment hybridized with nif Q-X probe and pNES 20 included the 5 Mdal foreign DNA fragment hybridized with nif NE and nif YK probe.

• Journal of Microbiology and Biotechnology
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• v.14 no.6
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• pp.1275-1279
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• 2004

Citron peel oil was extracted from citron (Citrus funas) fruit by steam distillation, and was used as starting material for microbial conversion to synthesize attractive flavor compounds by using Enterobacter agglomerans 6L. E. agglomerans was isolated from citron peel and was able to metabolize the citron peel oil and grew well ($A_{600}:\;3.0$) on the citron peel oil as the sole carbon source. Multiple terpene metabolites were produced by E. agglomerans 6L on M9 salt media with citron oil vapor. The identified bioconversion products from the citron peel oil included trans-2-decenal, octanol, $\delta$­valerolactone, $\gamma$-valerolactone, cryptone, hydroxycitronellol, cuminol, and $\gamma$-dodecalactone.

• Journal of the Korea Organic Resources Recycling Association
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• v.15 no.4
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• pp.125-130
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• 2007

Six bacteria, which showed the flocculating activity to food wastewater, were isolated from various environment. These strains were identified as Bacillus pumilus, Enterobacter sp., Pantotea agglomerans, Bacillus licheniformis, and two Bacillus sps. Among them, the flocculating activities of three strains, such as Enterobacter sp.(YK102), Bacillus sp.(YK103), and Pantotea agglomerans (YK104), were eight times or more higher than that of the control strain, Zoogloea ramigera. in the test with 0.5% kaolin. In the experiment with food wastewater, Enterobacter sp.(YK102) showed the highest flocculating activity which was 2.5 times higher than that of a control strain, Pseudomonas fluorescens.

• Microbiology and Biotechnology Letters
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• v.25 no.5
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• pp.490-494
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• 1997

A variety of microbial strains isolated from soil were tested for their ability to produce D-tagatose from D-galactose. An organism that can convert D-galactose into D-tagatose was selected and was identified as Enterobacter agglomerans. The cells grown on the induction medium containing 20 g/l arabinose were found to the best conversion potential among different carbohydrates and the conversion yield was about 15% when 20 gll galactose was used. The isolated crystals were obtained from the culture broth after the purification process such as treatment of ion resins, crystallization, and drying. The recovery yield was 70% after the purification. The crystals were identified as D-tagatose by the infrared spectroscopy, HPLC, specific optical rotation, and melting point.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.2
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• pp.189-195
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• 1997

A phosphate solubilizing bacterium (PSB) possessing a high ability to solubilize hydroxyapatite (HA) was isolated from the rhizosphere of wheat. The PSB markedly developed clear zones after inoculating for 36 hours at $30^{\circ}C$. This bacterium was identified as Enterobacter agglomerans through API 20E system and Biolog$^{TM}$ analysis. The values of similarity and distance coefficient from authentication trial of the strain were 0.656 and 4.79 respectively. High performance liquid chromatography (HPLC) of the products of this strain indicated that this strain excretes maily oxalic acid with som other organic acids. During the incubation period of E. agglomerans, the pH values showed an inverse correlation ($r^2=0.933^{**}$) with solubilization of inorganic phosphate. Acid phosphatase activity of the strain was 10-15 times greater than alkaline phosphatase activity. Alkaline phosphatase activity had almost constant near zero activity across time. The population of E. agglomerans greatly increased during the first day of inoculation ; however, it drastically decreased thereafter.

• Journal of Microbiology and Biotechnology
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• v.13 no.4
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• pp.636-639
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• 2003

Entercbacter agglomerans 6L was isolated from citron (Citrus junos) peel by using an enrichment culture containing (+)-limonene. It was able to metabolize limonene and grew well ($A_{600}$:4.5) on limonene as a sole carbon source. E. agglomernas 6L was highly resistant to limonene toxicity, and grew to 1.0 optical density ($A_{600}$) even at 5% (v/v) of limonene in Luria-Bertani media. ${\gamma}-Valerolactone$ and cryptone were detected as the major metabolic products of limonene by E. agglomerans 6L.

• Microbiology and Biotechnology Letters
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• v.16 no.5
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• pp.379-383
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• 1988

To evaluate the treatability of activated sludge induced by benzene with microorganisms, isolation and characterization of benzene-degrading microorganisms were carried out. Six bacterial isolates from the activated sludge were identified ; Pseudomonas fluorescens, Enterobacter agglomerans, Enterobacter cloacae, Klebsiella oxytoca, Citrobacter freundii and Klebsiella pneumoniae. P. fluorescens degraded 55% of benzene contained in the medium as a sole carbon source, E. cloacae 24%, E. agglomerans 41%, and K. oxytoca 32%. Optimal temperature, pH and benzene concentration for growth of P. fluorescens appeared to be 31$^{\circ}C$, pH 7.0, and 300mg benzene per liter. When the P. fluorescens was dominant in the activated sludge induced by benzene, the indicator protozoa was Aspidisca sp. When concentration of benzene was about 387mg per liter, the growths of Aspidisca sp. and Litonotus sp. were high. Protozoa, Litonotus sp. and Vorticella sp. did not grow over 1600mg of benzene per liter.

• Applied Biological Chemistry
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• v.32 no.3
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• pp.309-314
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• 1989

The cultural condition of Enterobacter agglomerans U-1, a polysaccharide producing soil bacterium, was examined. The optimal medium composition was that contains the following components per liter of distilled water; $sucrose(23.75\;g/L),\;peptone(2.06\;g/L),\;yeast\;extract(0.5\;g/L),\;KH_2PO_4(1.0\;g/L)$ and $MgSO_4{\cdot}7H_2O(1.0 g/L)$. The optimum temperature for polysaccharide production was $30^{\circ}C$, where 8.5 g/L of polysaccharide was produced. The apparent viscosity of fermentation broth after 3 days was 240 mPa.s. at $70sec^{-1}$. The product yield and specific productivity were 36% and 142.07 mg/g/h.

• Food Industry And Nutrition
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• v.2 no.1
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• pp.16-21
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• 1997

Microbial of enzymatical methods are suitable for production of rare monosaccharides. Using oxidation and reduction ability of Microorganisms, various rare ketoses and polyols can be produced, for example D-tagatose from galagtitol by Enterobacter agglomerans strain 221e. L-tagatose from galactitol by Klebsiella pheumonias strain 40b, L-psicose from allitol by Gluconobacter frateurii IFO 3254, D-talitol from d-tagatose by Aureobasidium pullulans strain 113B, allitol from D-psicose by Enterobacter agglomerans strain 221e and so on. We can produce various rare aldoses and ketoses using aldose isomerases, for example L-galactose from L-tagatose by D-arabnose isomerase, and L-ribose from L-ribulose by L-isomerase, and so on. D-Tagatose 3-epimerase of Pseudomonas sp. ST-24 is very useful for preparationof various rare ketoses, for example D-psicose from D-fructose, D-sorbose from D-tagatose, L-fructose, from L-psicose and so on. Using polyol dehydrogenases, aldose isomerases and D-tagatose 3-epimerase, we can design the suitable for production of a certain rare monosaccharide from a suitable substrate.