Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation of Angiotensin Converting Enzyme Inhibitors from Pig Blood

돼지혈액으로부터 항고혈압물질의 분리

  • Park, Eun-Hee (Department of Food Science and Technology, College of Agriculture, Chungnam National University) ;
  • Song, Kyung-Bin (Department of Food Science and Technology, College of Agriculture, Chungnam National University)
  • Published : 1997.02.28
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Abstract

Angiotensin converting enzyme (ACE) inhibitors were isolated and purified from pig blood plasma. Pig blood plasma was obtained after removing blood cells by centrfugation, followed by the addition of anticoagulant to whole pig blood. To precipitate plasma proteins, pig blood plasma was treated with 4% trichloroacetic acid (TCA) as a final concentration. ACE inhibitors were isolated from plasma protein hydrolysates and TCA supernatant, using ultrafiltration, gel permeation chromatography, and reverse-phase high pressure liquid chromatography. ACE inhibitors isolated from plasma hydrolysates and TCA supernatant had $IC_{50}$ values of $23\;{\mu}M$ and $2\;{\mu}M$, pentapeptide, respectively.

돼지혈액의 혈장으로부터 ACE 저해제들을 분리 정제하였다. 돼지 도축혈액을 먼저 혈액응고방지제를 첨가한후 원심분리에 의해 혈장과 혈구로 분리한 다음 혈장단백질을 최종농도 4% TCA로 침전시켰다. ACE 저해제들은 TCA 상등액과 혈장단백질 가수분해물로부터 한외여과, 겔여과크로마토그라피, 역상고속액체크로마토그라피 방법으로 분리되었는데 $IC_{50}4값들이 각각 $2\;{\mu}M,\;23\;{\mu}M$로 측정되었다.

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