The Effects of Allylibothiocyanate on the Biosynthesis of Metabolites of Aflatoxigenic Mold

Allylisothiocyanate 첨가가 Aflatoxin 생성 곰팡이 대사산물의 생합성에 미치는 영향

The effects of allylisothiocyanate on the biosynthesis of various fungus metabolites such as sterigmatocystin, lipid, protein, citrate RNA and AMP from the culture of Aspergillus Parasiticus R-716 were investigated. The content of sterigmatocystin, the precursor of aflatoxin, was lower in the culture added with 50ppm allylisothicoyanate after 48 hours, however was rather higher after 144 hours compared to that of the control. The addition of allylisothiocyanate resulted in the increase of lipid, protein, RNA in mycelium and the content of citrate in the media, but the amount of AMP was low.

무우에 함유된 allylisothiocyanate의 첨가가 Aspergillus parasiticus R-716의 배양시 sterigmatocystin, 지질, 단백질, RNA, citrate 및 AMP 등의 각종 대사산물의 생합성에 미치는 영향을 조사하였다. 그 결과 aflatoxin의 전구체인 sterigmatocystin의 함량은 배양 48시간 후에는 50ppm allyli-sothiocyanate 첨가구가 대조구보다 낮게 나타난 반면, 144시간째부터는 첨가구가 대조구보다 오히려 높게 나타났다. Allylisothiocyanate의 첨가로 Aspergillus parasiticus R-716의 배양액에서 citrate는 높은 함량을 나타내었으며, 또한 균체내 지질, 단백질, RNA의 함량은 높게 나타났으나 AMP 함량은 낮았다.