Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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Intestinal Permeabilities of Polyethylene Glycols (330-1122D) in the In Situ Perfused Rat

장내 관류된 동물에서 Polyethylene Glycols에 의한 장내 투과율 (Intestinal Permeability)측정에 관한 연구

  • 김미혜 (영국 Manchester 대학교, 약학과)
  • Published : 1996.03.01
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Abstract

Polyethylene glycols(PEGs)are hydrophilic molecules that have been used to characterize intestinal permeability via the paracellular pathway. Using a mixture of PEGs(400, 600 and 1000), containing oligomers in the molecular weight range 330 to 1122 D, the molecular weight permeability dependence in the jejunum of the rat small intestine was examined, employing an in situ recirculation perfusion technique. Individual oligomers were determined by HPLC with refractive detection. In the range studied, a distinct molecular weight cut-off was not apparent. Corrected for the length of jejunum used in the study, over the molecular weight range 330 to 1122D, the apparent permeability(Papp) of PEG ranged from 4.92$\pm$0.02$\times$10-5cm/sec(mean$\pm$SEM, n=5) to 0.28$\times$10-5cm/sec. Also, it was observed that the apparent permeability was inversely proportional to approximately MW2. The results in this study suggest that molecular weight is an important factor in determining the intestinal permeability.

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References

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