Journal of Periodontal and Implant Science
- Volume 25 Issue 3
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- Pages.694-702
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- 1995
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- 2093-2278(pISSN)
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- 2093-2286(eISSN)
THE EFFECT OF KOREAN RED GINSENG SAPONIN ON THE ALKALINE PHOSPHATASE ACTIVITY OF RAT OSTEOBLASTIC CELL(ROS17/2.8) IN CULTURE
한국 홍삼사포닌이 배양중인 쥐 조골세포의 염기성 인산분해효소 활성도에 미치는 영향
- Jung, Jin-Kwang (Dept. of Periodontology, School of Dentistry, Dankook University) ;
- Kim, Jung-Keun (Dept. of Periodontology, School of Dentistry, Dankook University) ;
- Lee, Jae-Hyoun (Dept. of Periodontology, School of Dentistry, Dankook University)
- Published : 1995.11.30
Abstract
Using the Korean red ginseng saponin, which is known to world-wide and thd effects of it have been investigated by many reserachers for years. Ginseng saponin, one of the major components of Korea ginseng root, has many various biologic effects, such as cytotoxic effect, tumorcidal activity, protein biosynthesis and membrane modifying effect. The purpose of this study was to evaluate effects of ginseng saponin on the alkaline phosphatase activity of ROS cells in culture. After ROS cells were seeded into a 96-well plate, 96-well plate cultured until confluence was obtained. To evaluate cytotoxic effect of total saponin in cultured ROS cells, the plates were added to each total saponin concentration (0-1mg/ml). After 48hr., cells were counted by stain with 0.2% trypan blue at randomly selected field microscopically. Also, to evaluate alkaline phosphatase(ALP) activity of total saponin in cultured ROS cell, the plate was added to each total saponin concentration (0-1mg/ml) and ALP activity was assayed. To evaluate time-course of ALP activity,