Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effect of Chronic Alcohol Feeding and 2-Acetylaminofluorene Treatment on Hepatic Mitochondrial ATPase Activity and Membrane Lipid Composition in Rats

만성 알코올 섭취시 2-Acetylaminofluorene 투여가 흰쥐 간 미토콘드리아 ATPase 활성도와 막지질 조성에 미치는 영향

  • 김정희 (서울여자대학교 영양학과) ;
  • 류선영 (서울여자대학교 영양학과)
  • Published : 1995.12.01
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Abstract

This study was done ot investigate the effect of chronic alcohol feeding and acetylaminofluorene(2-AAF) treatment on hepatic mitochondrial ATPase activity andmembrane lipid composition. Male Sprague-Dawley rats, weighing 120~125g, were fed for 6 weeks on a liquid diet containing 35% of calories as ethanol. After 4 weeks of experiment diet feeding, 2-AAF(100mg/kg body weight) was injected twice a week intraperitoneally. Body weight and percent liver weight per body weight were significantly changed by ethanol feeding. Hepatic mitochondrial ATPase activity significantly decreased by ethanol feedings but not by 2-AAF treatment. In comparison to control, the ATPase activity of ethanol-AAF group decreased 29.3%. Since phospholipid(PL) content of mitochondria has an interaction effect between ethanol and 2-AAF treatment, 2-AAF treatment significantly increased phospholipid content in only ethanol fed group. Total cholesterol(C) level of mitochondria significantly increased by ethanol feeding. Consequently C/PL ratio of ethanol group was significantly higher than that of control group. The analysis of mitochondrial PL composition showed that cardiolipin(CL) significantly increased by 2-AFF treatment in control group. Phosphatidyl choline(PC) significantly increased by ethanol feeding, whereas PC significanlty decreased and phosphatidyl ethanolamine(PE) significantly increased by 2-AAF treatment. 2-AAF treatment also showed a significant increase in PE/PC ratio. Fatty acid patterns of mitochondria were also changed by either ethanol or 2-AAF although the severity of the changes was not great. These data suggest that the reduced mitochondrial ATPase activity in ethanol-AAF group may be a consequence of a changes in mitochondrial membrane lipid composition such as PE/PC ratio, C/PL ration and fatty acid patterns.

만성적인 알코올 섭취가 미토콘드리아의 지질조성 및 ATPase 활성도에 미치는 영향을 조사하고 아울러 간의 발암물질인 2-AAF를 토여하여 간 미토콘드리아의 기능에 미치는 상호 효과를 조사하기 위하여 120~125g 숫쥐에게 열량의 35%를 알코올로 공급하여 6주간 사육하였으며 알코올 섭취 4주 후에 2-AAF를 3일 간격으로 2회 복강투여하여 실험한 결과를 요약하면 다음과 같다. 1. 체중 증가, 식이 섭취량 및 식이효율에 대한 알코올의 효과는 유의적인 차이를 보였으나, 2-AAF에 의한 유의적인 효과는 관찰할 수 없었다. 알코올 섭취군이 대조군 보다 체중은 유의적으로 감소하였다. 2. 미토콘드리아 ATPase 활성도는 알코올 섭취에 의해 유의적으로 감소되었고, 2-AAF 투여에 의해서는 유의적이지 않으나 감소되는 경향을 보였다. 따라서 대조군(C)에 비하여 알코올 섭취와 2-AAF투여군(EA)에서 ATPase 활성도가 29.3%의 감소를 나타냈다. 3. 간 미토콘드리아 콜레스테롤 함량은 알코올 섭취에 의해 유의적으로 증가하였으며 미토콘드리아 총 인지질량은 알코올과 2-AAF에 의한 상호작용에 의해 알코올 투여군에서만 2-AAF 투여시 유의적인 증가 효과를 보여주었다. C/PL 비율은 알코올 섭취와 2-AAF 투여 보두에서 유의적인 효과는 보이지 않았으나 군간 비교시 알코올군(E)이 대조군(C) 보다 유의적으로 증가하였다. 4. 간 미토콘드리아의 CL량은 알코올군에서 감소하는 경향을 보였고 PS와 PI량은 차이가 없었다. 알코올 섭취로 PC량은 유의적으로 증가하였으나 PE량은 차이가 없엇으며, 2-AAF 투여시 PC량은 유의적인 감소를 보였고 PE량은 유의적으로 증가하였다. PE/PC 비율은 알코올 투여에 의해 유의성은 없었으나 감소경향을 나타냈으며, 2-AAF 투여에 의해서는 유의적인 증가 효과가 나타났다. 5. 알코올 섭취시 미토콘드리아의 지방산 조성은 palmitic acid가 유의적으로 감소하였고, stearic acid는 유의적으로 증가함을 보였다. UFA/SFA와 UI는 알코올군에서 유의적이지는 않으나 증가되는 경향을 보였다. 2-AAF 투여에 의해서도 linoleic acid는 감소되고 arachidonic acid는 증가되는 경향을 보여 UI값이 증가되는 경향이 있었다. 따라서 본 실험에서 나타난 ATPase 호라성도 변화는 비토콘드리아의 콜레스테롤변화, C/PL 비율, 인지질 조성이나 지방산 조성의 변화 등 막지질 조성의 변화에 기인하는 것으로 생각된다.

Keywords

References

  1. JAMA v.249 Effect of alcohol intake on high density lipoprotein cholesterol levels in runners and inactive men Hartung,G.H.;Foreyt,J.P.;Michell,R.E.
  2. Medical and nutritional complications of alcoholism: mechanisms and management Alcohol and liver Lieber,C.S.;Leo,M.A.;Lieber,C.S.(ed.)
  3. J. Biol. Chem. v.245 Hepatic microsomal ethanol oxidizing system : In vitro characteristics and adaptive properties in vivo Liber,C.S.;DeCarli,L.M.
  4. J. Biol. Chem. v.252 Reconstitution of the microsomal ethanol-oxidizing system Ohnishi,K.;Lieber,C.S.
  5. DNA v.6 The P-450 gene superfamily : Recommended nomenclature Nebert,D.W.;Adesnik,M.;Coon,M.J.;Estabrook,R.W.;Gonzalez,F.J.;Guengerich,P.;Gunsalus,I.C.;Johnson,E.F.;Kemper,B.;Levin,W.;Phillips,I.R.;Sato,R.;Waterman,M.R.
  6. Biochem. Pharmacol. v.27 Introduction of microsomal N-hydroxylation of N-2-fluorenylacetamide in rat liver Maleijka,J.
  7. Cancer Res. v.41 Enhancement of dimethylnitrosamine metabolism and activation to a mutagen following chronic ethanol consumption Garro,A.J.;Seitz,H.K.;Lieber,C.S.
  8. Nutrition and the Origins of Disease Glutathione, alcohol and hepatotoxicity Kaplowitz,N.;Fernandez-Checa,J.;Ookhtens,M.;Halsted,C.H.(ed.);Rucker,R.B.(ed.)
  9. Biochem. Biophys. Res. Commun. v.143 Acetaldehyde-mediated hepatic lipid peroxidation : Role of superoxide and ferritin Shaw,S.;Jayatilleke,E.
  10. Arch. biochem. Biophys. v.191 Respective role of superoxide and hydroxy radical in the activity of the reconstituted microsomal ethanol oxidizing system Ohnishi,K.;Lieber,C.S.
  11. 이화여대의대지 v.7 알코올을 투여한 흰쥐에서 비타민 E 투여가 lipid peroxidation에 미치는 영향 홍영숙;함윤애;성낙응
  12. Nutr. Rev. v.46 The influence of alcohol on nutritional status Liber,C.S.
  13. Am. J. Clin. Nutr. v.33 Alcoholism and malnutrition. Introduction to the symposium Halsted,C.H.
  14. Nutr. Rev. v.45 Alcohol, vitamin A and zinc Leo,M.A.;Kim,C.;Lieber,C.S.
  15. Alcoholism : Clin. Exp. Res. v.10 The feeding of ethanol in Liquid diet Lieber,C.S.;Decarli,L.M.
  16. Alcohol & Alcoholism v.24 Liquid diet technique of ethanol administration 1989 update Lieber,C.S.;Decarli,L.M.
  17. Lipids v.8 Damage to microsomal membrane by lipid peroxidation Bidlack,W.T.;Tapple,A.L.
  18. J. Lab. Clin. Med. v.70 Studies on the quantitative and qualitative charaterization of erythrocyte glutathione peroxidase Paglia,E.D.;Valentine,W.N.
  19. Method in Enzymology v.52 Glutathione peroxidase and hydroperoxides Tappel,A.L.
  20. Methods in Enzymology Glutathione reductase Carlberg,I.;Mannervick,B.
  21. J. Biol. chem. v.249 Glutathione S-transferase Habig,W.H.
  22. J. Biol. chem. v.239 The carbon monoxide binding pigment of liver microsome Omura,T.;Sato,R.
  23. Anal. Biochem. v.75 Quantitative determination of cytochrome P-450 in rat liver homogenate Matsubura,T.;Koike,M.;Touchi,A.;Tochino,Y.;Sugeno,K.
  24. J. Biochem. v.193 Protein measurement with the folin phenol reagent Lowry,O.H.;Rosebrough,N.J.;Farr,A.L.;Randall,R.J.
  25. Alcoholism : Clin. and Exp. Res. v.6 The feeding of alcohol in Liquid diets : Two decades of applications and 1982 update Lieber,C.S.;Decarli,L.M.
  26. Gastroenterology v.84 Effect of hepatic vitamin A depletion on the liver in humans and rats Leo,M.A.;Sato,M.;Lieber,C.S.
  27. J. Nutr. v.111 Hepatic vitamin A depletion after chronic ethanol consumption in baboons and rats Sato,M.;Lieber,C.S.
  28. Nutr. Rev. v.36 Oxygen activation in the metabolism of lipids, drugs, and carcinogens Coon,M.J.
  29. Lancet v.2 Increased free radical activity in alcoholics Fink,R.;Marjot,D.H.;Cawood,P.;Iversen,S.A.;Clemens,M.R.;Patsalos,P.;Norden,A.G.;Dormandy,T.L.
  30. Dig. Dis. Sci. v.31 Depressed selenium and vitamin E levels in an alcoholic population : Possible relationship to hepatic injury through increased lipid peroxidation Tanner,A.R.;Bantock,I.;Hinks,L.;Lloyd,B.;Turner,N.R.;Wright,R.
  31. Exp. Molec. Pathol. v.8 No chemical evidence of hepatic lipid peroxidation in acute ethanol toxicity Hashmoto,S.;Recknagel,R.O.
  32. Rad. Biol. Med. v.7 Role of lipid peroxidation and oxidative stress in alcohol toxicity Cederbaum,A.I.
  33. Arch. Biochem. Biophys v.300 ESR studies on the production of reactive oxygen intermedlate by rat liver microsomes in the presence of NADPH or NADH Rashba-Step,J.;Turro,N.J.;Cederbaum,A.I.
  34. Ethanol 중독 흰쥐에서 총담관결찰이 간의 glutathione S-transferase, glutathione peroxidase, glutathione reductase 활성에 미치는 영향 v.23 곽춘식;김여희;조순승
  35. 한국영양학회지 v.23 BHT 첨가식이 및 2-AAF 투여가 식이지방을 달리한 쥐 간의 microsomal mixed function oxidase계에 미치는 영향 윤은영;최혜미
  36. Biochem. Biophys. Acta v.618 Rat hepatic cytosolic giutathione-dependent enzyme protection against lipid peroxidation in the NADPH microsomal lipid peroxidation system Burk,R.F.;Trumble,M.;Alawrence,R.
  37. Cancer Res. v.46 Effects of chronic ethanol consumption on benzo (a)pyrene metabolism and GST activity in Syrinn golden hamster check pouch and liver Murphy,S.E.;Hecht,S.S.
  38. Ann. Rev. Biochem. v.58 Glutathione S-transferase : Gene structure, regulation and biological function Pickett,C.B.
  39. Cancer Res. v.37 Ligandin,the glutathione S-transferase and chemically induced hepatocarcinogenesis : A review Smith,G.J.;Chl,V.S.;Litwack,G.
  40. 이화여자대학교 박사학위논문 알코올 투여가 흰쥐 간조직내 cytochrome 계 및 lipid peroxidation에 미치는 영향 유태화
  41. Cancer Res. v.48 Role of cytochrome P-450 enzymes in chemical carcinogenesis and cancer chemotherapy Guengerich,F.P.
  42. Cancer Res. v.38 Elevation of hepatic GST activity and protection against mutagenic metabolities of benzo (a)pyrene by dietary antioxidant Benson,A.M.;Batzinger,R.P.;Ou,S.Y.