Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Sequencing of Resistance Determinants to Aminoglycoside Antibiotics from Sterptoalloteichus hindustanus ATCC 31219

Streptoalloteichus hindustanus ATCC 31219로부터 아미노글라이코사이드계 항생제에 내성을 지정하는 유전자의 클로닝 및 염기서열 결정

  • 김종우 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 한재진 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 최영내 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 엄준호 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 윤성준 (동화약품공업주식회사 중앙연구소 생물공학연구실) ;
  • 현창구 (명지대학교 생명과학연구소) ;
  • 서주원 (명지대학교 생명과학연구소)
  • Published : 1995.08.01
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Abstract

Streptoalloteichus hindustanus ATCC 31219, a nebramycin complex producer, is similar to Streptomyeces tenebrarius in a viewpoint of resistance to a wide range of aminoglycoside antibiotics. S. tenebrarius has resistance mechanisms of 16s rRNA methylation and aminogycoside modification. However, it is not known whether resistance mechanisms of Stall. hindustanus are the same as in S. tenebrarius. Therefore, we have tried to isolate resistance determinants from Stall. hindustanus. Two different types of aminoglycoside resistance determinants were isolated from Stall. hindustanus and expressed in Streptomyces lividans TK24. The apramycin resistance gene (amr) and the tobramycin resistance gene (tmr) isolated from Stall. hindustanus showed broad resistance spectrum against a dozen of aminoglycoside antibiotics. The complete nucleotide sequences of apramycin resistance gene (amr) were determined. The deduced amino acid sequence of the amr gene of Stall hindustanus ATCC 31219 showed extensive sequence homology to the 16s rRNA methylase gene (kamB) of S. tenebrarius.

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References

  1. Industrial Microorganisms: Basic and Applied Molecular Genetics Baltz,R.H.;G.D.Hegeman;P.L.Skatrud
  2. Annu. Rev. Microbial. v.43 Organization and expression of genes involved in the biosynthesis of antibiotics and other secondary metabolites Martin,J.F.;P.Liras
  3. Mol. Gen. Genet. v.208 Gene cluster for streptomycin biosynthesis in Streptomyces griseus: Analysis of a central region including the major resistance gene Distler,J.;C.Braun;A.Ebert;W.Pipersberg
  4. Nucl. Acids Res. v.15 Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity Distler,J.;A.Ebert;K.Mansouri;K.Pissowotzki;M.Stockmann;W.Pipersberg
  5. Microbiol. Rev. v.57 Molecular gentics of aminoglycoside resistance genes and familial relationship of the aminoglycoside-modifying enzymes Shaw,K.J.;P.N.Rather;R.S.Hare;G.H.Miller
  6. J. Mol. Biol. v.193 Sites of action of two ribosomal RNA methylase responsible for resistance to aminoglycoside Beauclerk,A.A.D.;E.Cundliffe
  7. Genetic manipulation of Streptomyces Hopwood,D.A.;M.J.Bibb;K.F.Chater;T.Kieser;C.J.Bruton;H.M.Kiesser;D.J.Lydiate;C.P.Smith;J.M.Ward;H.Schrempf
  8. Molecular Cloning(2nd ed.) Maniatis,T.;J.Sambrook;E.F.Fritsch
  9. Mol. Gen. Genet. v.194 Involvement of 16S ribosomal RNA in resistance of the aminoglycoside producers Streptomyces tenjimariensis, Streptomyces tenebrarius and Micromonospora purpurea Piendl,W.;A.Bock;E.Cundliffe
  10. J. Gen. Microbiol. v.133 Cloning of aminoglycoside-resistance determinants from Streptomyces tenebrarius and comparison with related genes from other Actinomycetes Skeggs,P.A.;D.J.Holmes;E.Cundliffe
  11. Mol. Gen. Genet. v.200 Methylation of 16S ribosomal RNA and resistance to aminoglycoside antibiotics in clones of streptomyces lividans carrying DNA from Streptomyces tenjimariensis Skeggs,P.A.;J.Thompson;E.Cundliffe
  12. J. Antibiot. v.46 Analysis of the nucleotide sequence of fmrT endoding the selfdefense gene of the istamycin producer, Streptomyces tenjimariensis ATCC 31602: comparison with the sequences of kamB of Streptomyces tenebrarius NCIB 10028 and kamC of Saccharopolyspora hiruta Ohta,T.;M.Hasegawa