Korean Journal of Microbiology (미생물학회지)
- Volume 32 Issue 1
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- Pages.53-59
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- 1994
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Host Construction by Curing the Octopine Type Ti and Cryptic Plasmids in Agrobacterium tumefaciens KU12
Agrobacterium tumefaciens KU12로부터 Octopine형 Ti 및 잠재 플라스미드의 제거에 의한 숙주 개발
- Ha, Un-Hwan (Department of Biology, College of Science, Korea University) ;
- Lee, Yong-Woog (Department of Biology, College of Science, Korea University) ;
- Moon, Hye-Yeon (Department of Biotechnology, College of Engineering, Taegu University) ;
- Sim, Woong-Seop (Department of Biology, College of Science, Korea University)
- Published : 1994.01.01
Abstract
Agrobacterium tumefaciens KU12 contains pTiKU12 (240kb) of the octopine type Ti plamsid and pTi12 (45 kb) of the cryptic plasmid. To make the avirulent A. tumefaciens, the octopine type Ti plasmid, pTiKU12, was cured with elevated temperature (37
Agrobacterium tumefaciens KU12내에 존재하는 240kb 크기으 octopine형 Ti 플라스미드인 pTiKU12와 45kb 크기의 잠재 플라스미드인 pTi12를 제거하여 무독성의 A. tumefaciens 균주를 제조하였다. Octopine형 Ti 플라스미드인 pTiKU12는 고온(37