Aspergillus sp. BY-54가 생산하는 Dextranase의 정제 및 특성

  • 방병호 (서울보건전문대학 식품영양과) ;
  • 이진영 (서울보건전문대학 식품영양과)
  • 발행 : 1994.06.01

초록

Aspergillus sp. BY-54 which produced a strong dextran hydrolyzing enzyme was isolated from soil. Using this strain, the optimal cultural conditions, enzyme purification and characterization were studied. The results are as follows : The optimal concentration of dextran as carbon source was l%. and the optimum temperature and the initial pH for enzyme production was 3$0^{\circ}C$, and 7.0, respectively. Dextranase was purified by DEAE-cellulose column chromatography with a linear gradient increase in NaCl. Km value of dextranase was 0.222%, and several glucans containing various types of glucosidic linkages such as DEAE-sephadex, CM-sephadex and sephadex G-100 were almost digested to a large extent with this dextranase. The enzyme was strongly inhibited by sodium fluoride, KMnO4 and p-CMB, while KCN caused 20% of activation.

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