Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Reisolation Frequency of Soil Actinomycetes on Multiple Isolation Media

다수 배지의 사용에 따른 방선균의 중복 분리빈도

  • 김창진 (한국과학기술연구원 유전공학연구소) ;
  • 이강현 (한국과학기술연구원 유전공학연구소) ;
  • 아끼라 시마즈 (동경대학교 분자세포생물학연구소) ;
  • 유익동 (한국과학기술연구원 유전공학연구소)
  • Published : 1994.06.01
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Abstract

For effective screening research, to isolate many different strains not duplicated would be essential. Three kinds of media were used for selective isolation of actinomycetes to test the reisolation frequency. Sixty species were isolated on Bennet's agar, 47 were on glycerol-asparagine agar and 77 were on humic acid-vitamin agar from 10 domestic soil samples. All of these isolates were identified to the genus level based on morphological characters and examined the sameness on each medium. The reisolation frequency between two different media was 10% or so(8.3~16.7%) and among three different media was 25% or so(22.1~27.7%).

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References

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