Purification of Extracellular Enzyme Produced by Vibrio sp. AL-145

Vibrio sp. AL-145가 생산하는 균체외 효소의 정제 (I)

The alginate degrading bacteria have been screened from the marine environment. Sodium alginate and NaCl were required for cell growth and enzyme production of 145-C strain and the adequate concentrations were 0.7 and 2.5%, respectively. The effective nitrogen source was peptone and adequate temperature was 28$\pm$2$^{\circ}C$. The 145-C strain was identified as Vibrio sp. from biochemical and biological experiment. The extracellular enzyme produced by Vibrio sp. was purified and the molecular weight was estimated to be 27, 000.

알긴산 분해능이 강한 균주를 자연산 미역으로부터 분리하여 동정한 결과 Vibrio sp.로 밝혀졌고, 이 균은 탄소원으로 alginate, 질소원으로 peptone, NaCl 농도 2.5%, 28$\pm$2$^{\circ}C$에서 최대의 효소활성을 보였다. 겔 여과 및 이온크로마토그래피 방법으로 정제하여 정제도가 53.7배, 비활성이 11.84U/mg의 정제효소를 얻었다. 이 정제효소를 SDS-전기영동하여 분자량을 측정한 결과 약 27,000 정도로 추정되었다.