Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Biological Activities of MT 1155 Inducing Morphological Change of Rous Sarcoma Virus-Transformed Normal Rat Kidney Cell

Rous Sarcoma Virus에 의해 형질전환된 NRK 세포의 형태변화를 유도하는 활성물질 MT 1154의 분리와 생물학적 활성

  • 안종석 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 박문수 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 박찬선 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 민태익 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 안순철 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 오원근 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 이현선 (한국과학기술연구원 유전공학연구소 미생물공학연구실) ;
  • 윤병대 (한국과학기술연구원 유전공학연구소 미생물공학연구실)
  • Published : 1993.02.01
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Abstract

We isolated Actinomycetes strain GMT 1155 and purified the active compound, MT 1155, on the morphological reversion of ts/NRK cell from the isolate. MT 1155 was identified as toyocamycin having antifungal and antitumor activities from physico-chemical properties and UV, IR, $^1H$-NMR, $^13C$-NMR and mass spectrum. MT 1155 showed the morphologically reversional activity on ts/NRK cell and the cytotoxicity on CTLL cell at the final concentrations of 1.7 JlM and 0.2 11M, respectively and its $IC_{50}$ value on protein kinase A enzyme was 2.3 $\mu$M. Also it had strong antifungal activity against several pathogenic fungi but not antibacterial activity. And it did not inhibit both protein kinase C activity and the bleb-formation of K562 cell induced by phorbol esters.

방선균 분리주 GMT 1153로부터 ts/NRK 세포의 형태를 변형된 세포형태에서 정상세포의 형태로 전환하는 활성물질을 분리, 정제하였다. 활성물질 MT 1155는 UV, IR, 1H-NMR, 13C-NMR, mass,원소분석 등의 기기분석을 통하여 항진균성 항암물질인 toyocamycin으로 동정되었다. MT 1155는 ts/NRK 세포의 형태전환에 대한 활성외에도 PKA 효소활성 저해와 CTLL 세포에 대한 세포독성이 있으나 K562 소포형성 억제 및 PKC 효소활성에 대한 저해효과도 없었다.

Keywords

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