Applied Biological Chemistry
- Volume 33 Issue 2
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- Pages.169-173
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- 1990
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Inhibitory effects of grapefruit seed extract(DF-100) on growth and toxin production of Penicillium islandicum
자몽종자 추출물(DF-100)이 Penicillium islandicum생육 및 독소 성분 skyrin생합성에 미치는 저해효과
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Cho, Sung-Wan
(Dept of Food Technology, Gyeongsang National University)
;
- Seo, Il-Won (ABCON CHEMIE Co., Ltd.) ;
- Choi, Jong-Duck (Dept of Food Technology, National Tongyong Fisheries Technical College) ;
- Joo, In-Saeng (Dept of Food Technology, Gyeongsang National University)
- Published : 1990.06.30
Abstract
The anthraquionone mycotoxin, skyrin, is produced by Penicillium islandicum. DF-100 which was extracted from grapfruit seed extract and is a natural organic complex inhibited the biosynthesis of skyrin by Penicillium islandicum. This study was carried out to determine the potential of DF-100 to support Penicillium islandicum and skyrin production. DF-100 inhibited the growth of the fungus at 750ppm or less and caused complete inhibition of skyrin production at 500ppm or less. DF-100 appears to block the incorporation of emodinathrone into skyrin and an enzymatic step in the skyrin biosynthetic pathway which lies before skyrinanthrone.
곡류 등에 오염되어 황색 독소성분인 skyrin을 생합성하는 Penicillium islandicum 배지에 grapefruit 종자로부터 추출한 천연유기 복합물인 DF-100을 처리하여 곰팡이 생육 및 skyrin 생성을 저해하는 효과를 볼 수 있었다. DF-100 500ppm 농도로 Penicillium islandicum의 생육을 91% 저해하였고 750ppm 농도에서 곰팡이 생육을 완전히 저해하였으며 500ppm농도로 skyrin생성을 완전히 저해하였다. skyrin 생합성경로를 고려할 때 DF-100은 낮은 농도에서 emodinanthrone으로부터 emodin을 거쳐 skyrin으로 전환되는 효소 반응계와 skyrinanthrone으로 진행하는 반응단계를 저해하는 것으로 밝혀 졌다.