세포증식과 증식속도의 On-line Monitoring을 위한 Computer- coupled Mass Spectrometer의 응용

Application of Computer-coupled Mass Spectrometer for Continuous On-line Monitoring of Cell Growth and Growth Rate

Quadrupole mass spectrometer를 이용한 발효 배기가스의 분석을 통해 세포의 증식을 on-line monitoring하고자 model 균주로 Candida utilis에 대해 연구하였다. Quadrupole mass spectrometer와 interface된 16-bit 개인용 컴퓨터 (IBM PC-AT)에서 산소 소비속도(OUR)와 이산화탄소 발생속도(CER)를 on-line 계산할 수 있었고 계속해서 이들 계산치로부터 세포농도와 증식속도 및 비증식속도를 계산하였다. 계산된 값들은 실험적으로 측정한 세포농도 및 비증식속도와 잘 일치함을 알 수 있었다.

Continuous on-line monitoring of cell concentration and growth rate in aerobic batch fermentation process was carried out by analyzing the exhaust gas composition of tormentor with a quadrupole mass spectrometer. From the mass spectrometric analyses of major gaseous components, i.e. $N_2$, $O_2$, $CO_2$ and $H_2O$, and the material balance equations for oxygen and carbon dioxide, oxygen uptake rate (OUR) rind carbon dioxide evolution rate (CER) were instantaneously calculated using a computer (16-bit IBM PC-AT) interfaced to a quadrupole mass spectrometer. The calculated OUR and CER data were used for the estimation of cell concentration and growth rate of Candida utilis during batch culture. It was found that the cell concentration could be satisfactorily estimated from the data of OUR arid CER during the culture and this method could be successfully und for the continuous monitoring of cell growth and growth rate.